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目的对分离的人博卡病毒WLL-1株进行全基因组测序和生物信息学分析。方法运用NCBI提供的blastp分析各编码蛋白质的同源性;用SIB提供的ProtParam和ScanProsite推测计算蛋白质的各种物理特性参数。蛋白进行结构域分析采用SIB提供的ScanProsite。对具有同源序列和无同源序列的蛋白分别采用SwissProt和Scratch Protein Predictor预测其三级结构。蛋白质功能分析采用CBS Prediction Servers提供的ProtFun软件。结果WLL-1病毒株与其它微小病毒不存在亲缘关系,与目前报道的其他几株人博卡病毒有高度同源的基因组序列。WLL-1病毒株与其它已报道的病毒株有一定的进化距离,具有一些独特的突变位点。在NS1蛋白中发现调节DNA复制的超家族3结构域,在衣壳蛋白VP1、VP2中发现了在病毒的自组装中起重要作用的“β桶”拓扑基序。发现NP1蛋白具有多个N-糖基化及磷酸化修饰位点。结论经基因顺序分离病毒为人博卡病毒,这些位点参与病毒基因的翻译调控。
OBJECTIVE: To perform genome-wide sequencing and bioinformatics analysis of isolated human Baka strain WLL-1. Methods Blastp provided by NCBI was used to analyze the homology of each encoded protein. ProtParam and ScanProsite provided by SIB were used to calculate various physical characteristic parameters of protein. Protein profiling was performed using ScanProsite provided by SIB. The tertiary structure was predicted by SwissProt and Scratch Protein Predictor respectively for proteins with homologous sequences and without homologous sequences. Protein functional analysis using ProtFun software provided by CBS Prediction Servers. Results There was no genetic relationship between the WLL-1 strain and other parvoviruses, which was highly homologous to several other reported human Boka strains. The WLL-1 strain has some evolutionary distance from other reported strains and has some unique mutation sites. The discovery of a superfamily 3 domain that regulates DNA replication in the NS1 protein revealed a “β-barrel” topology motif that plays an important role in viral self-assembly in the capsid proteins VP1 and VP2. The NP1 protein was found to have multiple N-glycosylation and phosphorylation sites. Conclusion The virus was isolated by gene sequence as human Boka, these sites involved in translational regulation of the viral genes.