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【目的】对2年生酸枣移栽苗进行耐盐性评价,为其在新疆盐渍化地区扩大种植面积提供依据。【方法】以2年生酸枣移栽苗为材料进行不同程度的NaCl胁迫处理,研究其细胞膜透性、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和渗透调节物质含量的变化趋势。【结果】将2年生酸枣幼苗移栽后第80 d(CK1),其叶片细胞膜透性、SOD、POD、CAT活性和脯氨酸含量均显著高于2年生酸枣实生苗(CK)(P<0.05);MDA、可溶性糖、淀粉、蛋白质含量没有达到显著水平(P>0.05)。NaCl胁迫下,浓度为0～200 mmol/L,2年生酸枣移栽苗叶片中细胞膜透性,SOD、POD、CAT活性显著高于2年生酸枣实生苗(CK)(P<0.05);浓度为250、300 mmol/L时,SOD、POD的活性下降,而CAT活性处于稳定状态。随之NaCl浓度的加重,2年生酸枣移栽苗叶片中脯氨酸的含量成倍增加,可溶性糖、可溶性蛋白、淀粉的含量均呈增加趋势。【结论】对于酸枣移栽苗来说,在NaCl浓度为0～200 mmol/L,其叶片中的活性氧清除酶开始启动,解除活性氧的毒害,脯氨酸起着重要的渗透调节作用,因此,在此范围内的酸枣移栽苗受到盐害较轻;而在250～300 mmol/L的NaCl胁迫下,酸枣移栽苗受到的伤害比较高。
【Objective】 Salt tolerance evaluation of 2-year-old jujube jujube transplanting seedlings was carried out to provide basis for expanding planting area in salinized areas of Xinjiang. 【Method】 Two-year-old Chinese jujube (Ziziphus jujuba Mill.) Transplanted seedlings were treated with different degrees of NaCl stress to study their cell membrane permeability, malondialdehyde (MDA) content and superoxide dismutase (SOD), peroxidase (POD) , Catalase (CAT) activity and osmotic adjustment substance content trend. 【Result】 The results showed that the leaf membrane permeability, activities of SOD, POD, CAT and proline in leaves of 2-year-old jujube seedling were significantly higher than those of 2-year jujube seedling (CK) after transplanted for 80 days (P < 0.05); MDA, soluble sugar, starch, protein content did not reach significant level (P> 0.05). Under NaCl stress, the membrane permeability, the activities of SOD, POD and CAT in 2-year-old jujube leaves were significantly higher than those in 2-year jujube seedlings (CK) (P <0.05) at concentrations of 0-200 mmol / L; At 250 and 300 mmol / L, the activity of SOD and POD decreased while the activity of CAT was steady. With the aggravation of NaCl concentration, the content of proline in seedlings of 2-year-old jujube jujube seedlings increased exponentially, and the contents of soluble sugar, soluble protein and starch increased. 【Conclusion】 For the transplanted jujube, the active oxygen scavenger started to activate in the NaCl concentration range of 0 ~ 200 mmol / L, releasing the poisoning of reactive oxygen species. Proline plays an important role in osmotic adjustment. Therefore, in this range of jujube transplanted seedlings suffered salt damage less; and 250 ~ 300 mmol / L of NaCl stress, jujube transplanting seedlings suffered relatively high damage.