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本文报道用40:B1 McAb检测曼氏血吸虫感染者血或尿中血吸虫成虫循环阴极(M)抗原具高度特异性。用曼氏血吸虫成虫排泄/分泌抗原(ESA)(含循环阴极(M)抗原)免疫的BALB/c小鼠的脾细胞与SP2/0骨髓瘤细胞融合,获得数株能识别阴极(M)抗原的McAb。用IFA分别定位于成虫肠道、体部和皮层,抗体亚类分别为IgG_3,IgM或IgG_1。分析了其中一株McAb 40:B1(IgG_3,用IFA定位于成虫肠道和体部)检测血吸虫循环抗原的特异性。
This paper reports the use of 40: B1 McAb to detect Schistosoma mansoni blood or urine of adult worms circulating antigen (M) has a high degree of specificity. Spleen cells from BALB / c mice immunized with adult Schistosoma japonicum adult excretion / secretion antigens (ESA) (including circulating antigen (M) antigen) were fused with SP2 / 0 myeloma cells to obtain several strains that recognized the antigen of the cathode (M) Of McAb. The IFA was located in the intestine of adult, body and cortex, the antibody subclasses were IgG_3, IgM or IgG_1. The specificity of one McAb 40: B1 (IgG_3, located in the intestine and body of adult adults with IFA) for the detection of circulating antigens in Schistosoma japonicum was analyzed.