长时间失血性休克家兔血浆一氧化氮活性测定及其意义

• 作 者: 任成山 陆海华 胡云 冯连荣 郭丽霞 郭中杰 高全杰
• 机 构: 第三军医大学附属新桥医院急诊科
• 出 处: 中国急救医学
• 发表日期: 1999年02期
• 关键词: 失血性休克 血浆一氧化氮 休克.失血性 有显著性差异 扩张血管 血管阻力 舒张血管 不可逆性休克 病理性改变 脂质过氧化

目的为探讨一氧化氮（ＮＯ）在失血性休克中的效应及其意义。方法复制家兔失血性休克模型，动态监测血浆亚硝酸盐（ＮＯ－２）活性，以此作为ＮＯ含量，同时分别测定血浆丙二醛（ＭＤＡ）浓度和超氧化物歧化酶（ＳＯＤ）含量。结果Ａ组动物于休克后ｌｈ血浆ＮＯ－２活性最高，与组间休克前和Ｂ同时相比较有显著性差异（Ｐ＜００５）。血浆ＳＯＤ含量于休克后ｌｈ和ＭＤＡ浓度休克后４ｈ和８ｈ最高。Ａ组动物于休克后ｌｈ血浆ＮＯ－２含量与同时相ＳＯＤ含量呈正相关（ｒ＝０６３７，Ｐ＜００５），与４ｈ和８ｈ同时相ＭＤＡ含量均呈正相关（ｒ分别为０６４０和０６４７，Ｐ＜００５），与休克后１ｈ同时相血压呈负相关（ｒ＝－０７２５，Ｐ＜００１）。结论失血性休克时过量产生的ＮＯ可直接扩张血管，降低血管阻力，使血压进一步下降。血浆ＮＯ－２、ＭＤＡ和ＳＯＤ浓度三者之间在休克时的关系，其确切机制目前尚不十分清楚，有待进一步深入研究

Objective To investigate the effect and significance of nitric oxide (NO) in hemorrhagic shock. Methods Rabbit hemorrhagic shock model was duplicated and the activity of plasma nitrite (NO-2) was dynamically monitored. The content of NO was measured and the content of plasma malondialdehyde (MDA) and the content of superoxide dismutase (SOD) were measured respectively. Results The plasma NO-2 level in group A was the highest at 1 h after shock, which was significantly different from that before shock and at group B (P <005). Plasma SOD content in shock after lh and MDA concentrations shock 4h and 8h after the highest. There was a positive correlation between plasma NO-2 level and SOD level in group A at 1h after shock (r = 0637, P <005), and positive correlation with MDA content at 4h and 8h (r = 00 640 and 0647, respectively, P <005), which was negatively correlated with the same blood pressure at 1 hour after shock (r = -0.725, P <001). Conclusion Overproduction of NO during hemorrhagic shock can directly dilate blood vessels, decrease vascular resistance and further decrease blood pressure. The exact mechanism of the relationship between plasma concentrations of NO-2, MDA and SOD at the time of shock is not yet clear and needs further study