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目的探讨单-ADP-核糖基转移酶3(mono-ADP-ribosyltransferase 3,ART3)过表达对人乳腺癌MDA-MB-231细胞增殖、凋亡及侵袭的影响。方法在Lipofectamine 2000的介导下,将ART3基因重组真核表达质粒pCMV-ART3-myc-tag转染人乳腺癌细胞MDA-MB-231,用G418进行稳定转染细胞株的筛选,同时设未转染组对照。Western blot法检测稳定转染细胞中ART3-myc-tag蛋白的表达,MTT法、流式细胞术和Transwell试验分别检测ART3对MDA-MB-231细胞增殖活力、凋亡及侵袭能力的影响。结果 ART3可在MDA-MB-231细胞中稳定过表达;ART3转染组细胞的增殖活力和侵袭能力均明显高于未转染组(P<0.05);ART3转染组细胞的凋亡率明显低于未转染组(P<0.05)。结论过表达ART3能促进乳腺癌细胞增殖、侵袭,并抑制其凋亡,本实验为进一步研究ART3基因在乳腺癌发生发展中的作用及其机制奠定了基础。
Objective To investigate the effects of mono-ADP-ribosyltransferase 3 (ART3) overexpression on the proliferation, apoptosis and invasion of human breast cancer MDA-MB-231 cells. Methods Human breast cancer cell line MDA-MB-231 was transfected with ART3 gene recombinant plasmid pCMV-ART3-myc-tag by Lipofectamine 2000. The stable transfected cell lines were selected by G418, Transfection group control. The expression of ART3-myc-tag in stable transfected cells was detected by Western blot. The effects of ART3 on proliferation, apoptosis and invasion of MDA-MB-231 cells were detected by MTT assay, flow cytometry and Transwell assay. Results ART3 could stably overexpressed in MDA-MB-231 cells. The proliferation and invasion ability of ART3 transfected cells was significantly higher than that of untransfected cells (P <0.05). The apoptosis rate of ART3 transfected cells was significantly Lower than the untransfected group (P <0.05). Conclusion Overexpression of ART3 can promote the proliferation, invasion and apoptosis of breast cancer cells. This study laid the foundation for the further study on the role and mechanism of ART3 gene in the development of breast cancer.