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目的探讨不同方法在肠道病毒71型(EV71)检测中的效果对比分析,方便对EV71做出及时而有效的诊断,为该病快速鉴别诊断奠定基础。方法采用ELISA、荧光定量RT-PCR、RT-PCR 3种方法对EV71病毒特异性和灵敏度进行综合分析。结果通过对比分析,ELISA方法检出阳性18份,阳性率为11.32%;实时荧光定量PCR方法检出阳性53份,阳性率为33.33%;RT-PCR方法检出阳性51份,阳性率为32.08%。结论 3种方法中实时荧光定量PCR方法具有较高的灵敏度及特异性,更具优势,更能满足临床需要。
Objective To explore the comparative analysis of different methods in the detection of enterovirus 71 (EV71) in order to facilitate the timely and effective diagnosis of EV71 and lay a foundation for the rapid differential diagnosis of the disease. Methods The specificity and sensitivity of EV71 virus were analyzed by ELISA, fluorescence quantitative RT-PCR and RT-PCR. Results By contrast analysis, 18 positive samples were detected by ELISA, the positive rate was 11.32%. The positive rate was 33.33% by real-time fluorescence quantitative PCR and 51 positive by RT-PCR, the positive rate was 32.08 %. Conclusion The real-time fluorescence quantitative PCR in the three methods has higher sensitivity and specificity, more advantages, and can better meet the clinical needs.