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目的 研究 survivin在肝细胞癌 (HCC)中的表达及其与肝癌增殖活性的相关意义。方法 应用免疫组织细胞化学技术检测 31例肝细胞癌组织 ,8例肝硬化组织和 4例正常肝组织中survivin及增殖细胞核抗原 (PCNA)的表达水平。结果 正常肝组织和肝硬化组织中均无 survivin表达 ,31例肝细胞癌组织中 19例 (6 1.3% ) survivin表达阳性。与正常肝组织和肝硬化组织相比 ,survivin在肝癌组织中表达率显著升高 (P <0 .0 0 1)。低分化肝癌其 survivin表达阳性率显著高于高分化肝癌。此外 ,survivin表达与肝癌增殖活性显著相关 ,survivin阳性肿瘤具有较高的增殖活性 ,其 PCNA指数 30 .5 %明显高于 survivin表达阴性肿瘤 15 .8% (P <0 .0 5 )。结论 survivin在肝癌中呈现高表达 ,这种特异性表达与肝癌的低分化性和高增殖有关 ,可能涉及到肝癌的发生和进展。survivin基因有望成为肝癌诊断和治疗的新靶点
Objective To study the expression of survivin in hepatocellular carcinoma (HCC) and its correlation with the proliferative activity of hepatocellular carcinoma (HCC). Methods The expressions of survivin and proliferating cell nuclear antigen (PCNA) in 31 cases of hepatocellular carcinoma, 8 cases of cirrhosis and 4 cases of normal liver tissue were detected by immunohistochemistry. Results There was no survivin expression in normal liver tissues and cirrhosis tissues, and 19 of 31 HCC tissues (6 1.3%) showed positive expression of survivin. Compared with normal liver tissues and cirrhotic tissues, survivin expression was significantly increased in HCC tissues (P <0.01). The positive rate of survivin in poorly differentiated HCC was significantly higher than that in HCC. In addition, the expression of survivin was significantly associated with the proliferation of hepatocellular carcinoma. Survivin-positive tumors showed high proliferative activity with a PCNA index of 30.5% which was significantly higher than that of survivin-negative tumors (15.8%, P <0.05). Conclusions Survivin is highly expressed in hepatocellular carcinoma. This specific expression is related to the poorly differentiated and hyperplastic hepatocellular carcinoma, which may be related to the occurrence and progression of hepatocellular carcinoma. Survivin gene is expected to become a new target for the diagnosis and treatment of liver cancer