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中枢5-羟色胺(5-HT)可通过兴奋下丘脑-垂体-甲状腺轴加重大鼠冷冻加束缚应激性溃疡。本工作进一步观察了脑室注射5-HT对胃粘膜代谢的影响及其与甲状腺激素的关系。应激180min大鼠的胃粘膜ATP酶的活性较非应激发对照鼠升高42.6％;如应激前10min向侧脑室注射5-HT50μg或腹腔注射四碘甲腺原氨酸(T_4)200μg/kg,均匀进一步提高应激后胃粘膜ATP酶的活性。在侧脑室注射5-HT后应激180min的鼠,血浆三碘甲腺原氨酸(T_3)和T_4水平均与胃粘膜ATP酶的活性呈明显的正相关关系。用高效液相层析测定观察到,应激鼠胃粘膜ATP和ADP水平均明显低于非应激鼠,分别为非应激鼠的70.3％和76.8％。腹腔注射T_4进一步减少应激后胃粘膜ATP和ADP的水平。这些结果提示,中枢5-HT可能通过甲状腺激素提高胃粘膜ATP酶的活性,使ATP和ADP含量下降,这可能与其加重溃疡的机制有关。
Central serotonin (5-HT) may increase the rat’s freezing and restraining stress ulcer by excitement of the hypothalamus-pituitary-thyroid axis. This work further observed the impact of intraventricular injection of 5-HT on gastric mucosal metabolism and its relationship with thyroid hormone. Stress gastric mucosal ATPase activity of 180min rats increased 42.6% than non-stressed control mice; 10 minutes before the stress into the lateral ventricle injection of 5-HT50μg or intraperitoneal injection of tetraiodothyronine (T_4) 200μg / kg, even further increase gastric mucosal ATPase activity after stress. The level of T_3 and T_4 in plasma of rats that were stressed 180 minutes after 5-HT injection into the lateral ventricle was positively correlated with the activity of ATPase in gastric mucosa. It was observed by HPLC that the levels of ATP and ADP in gastric mucosa in stress rats were significantly lower than those in non-stressed rats, which were 70.3% and 76.8% of those in non-stressed rats, respectively. Intraperitoneal injection of T_4 further reduces gastric mucosal ATP and ADP levels after stress. These results suggest that central 5-HT may increase gastric mucosal ATPase activity through thyroid hormones and decrease the content of ATP and ADP, which may be related to its mechanism of aggravating ulcer.