论文部分内容阅读
目的：探讨各种嗜酸细胞活化因子对支气管哮喘患者末梢血嗜酸细胞表面粘附分子表达的影响。方法：嗜酸细胞分离采用Ｐｅｒｃｏｌ不连续比重梯度离心法，粘附分子测定采用流式细胞仪ＦＡＣＳｃｎａ。结果：血小板活化因子（ＰＡＦ）、Ｐ物质（ＳＰ）、神经激肽Ａ（ＮＫＡ）、卟啉醇肉豆蔻酸乙酸酯（ＰＭＡ）及粒—巨噬细胞集落刺激因子（ＧＭ—ＣＳＦ）均具有增强嗜酸细胞表面粘附分子ＬＦＡ１β及Ｍａｃｌ表达的作用（Ｐ＜０．０１）；而ＶＬＡ４，ＬＥＣＡＭ—Ｉ于ＰＡＦ、ＧＭ—ＣＳＦ、ＰＭＡ及ｆＭＬＰ刺激后明显降低（Ｐ＜０．０１），ＩＬ－４刺激后无变化。结论：ＰＡＦ、ＳＰ、ＮＫＡ、ＧＭ—ＣＳＦ及ＰＭＡ作为嗜酸细胞活化因子通过增强其表面粘附分子ＬＦＡ１β及Ｍａｃｌ的表达在支气管哮喘病理形成上发挥着重要作用
Objective: To investigate the effect of various eosinophil activation factors on the expression of adhesion molecules on the surface of eosinophils in patients with bronchial asthma. Methods: The eosinophils were separated by Percol discontinuous gravity gradient centrifugation and the adhesion molecules were determined by flow cytometry FACScna. Results: PAF, SP, NKA, PMA and GM-CSF (P <0.01). However, VLA4 and LECAM-I significantly decreased after stimulation with PAF, GM-CSF, PMA and fMLP (P <0.01), and increased the expression of LFA1β and Macl in eosinophils No change after IL-4 stimulation. CONCLUSION: PAF, SP, NKA, GM-CSF and PMA, as eosinophil activators, play an important role in the pathological development of bronchial asthma by enhancing the expression of their surface adhesion molecules LFA1β and Macl