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目的探讨2型糖尿病(T2DM)合并微血管并发症患者的DNA氧化损伤。方法将87例T2DM患者分为糖尿病微血管并发症(A)组(n=56)和T2DM无微血管并发症(B)组(n=31),另选65例正常体检者为正常对照(C)组。用ELISA方法测定血液单核细胞DNA氧化损伤指标-8羟基脱氧鸟嘌呤(8-OHdG)水平。结果A、B组血液8-OHdG水平均较C组显著升高;A组病程、24h尿白蛋白、血液8-OHdG水平也较B组显著升高。结论T2DM合并微血管并发症患者的DNA氧化损伤较正常对照及无并发症糖尿病患者明显,对糖尿病合并微血管并发症者施行抗氧化治疗是合理的。
Objective To investigate DNA oxidative damage in patients with type 2 diabetes mellitus (T2DM) complicated with microvascular complications. Methods Eighty-seven patients with T2DM were divided into diabetic microangiopathy (group A) (n = 56) and T2DM without microvascular complication (group B) (n = 31). Another 65 normal subjects were selected as normal control (group C) group. The level of 8-OHdG, an indicator of DNA oxidative damage in blood mononuclear cells, was measured by ELISA. Results The level of 8-OHdG in group A and group B was significantly higher than that in group C. The duration of group A, the level of urinary albumin in 24h and the level of 8-OHdG in blood were also significantly higher than those in group B. Conclusion DNA oxidative damage in patients with T2DM complicated with microvascular complications is more obvious than that in normal controls and without complications, and it is reasonable to perform anti-oxidative therapy on diabetic patients with microvascular complications.