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以2年生大花月季品种“黑魔术”“金奖章”“全美小姐”当年生枝条为试材,采用电阻抗图谱(EIS)法研究了茎在4—8月的耐热性与9月至翌年1月的抗寒性,并对高温、低温胁迫下电阻抗参数的变化进行了详细研究,以期确定各电阻抗参数对温度胁迫的响应,并得到不经温度处理估测半致死温度的最适参数及响应回归方程。结果表明:胞外电阻率re和弛豫时间τ随高温、re随低温处理的变化曲线能用Logistic方程得到较好拟合结果;不经温度处理样本最适估测高温半致死温度的参数分别为“黑魔术”胞内电阻率ri(r=0.795)、“金奖章”ri(r=0.809)、“全美小姐”τ(r=-0.858);最适估测低温半致死温度的参数分别为“黑魔术”弛豫时间发布系数ψ(r=0.967)、“金奖章”re(r=-0.873)、“全美小姐”ψ(r=0.922)。
The annual shoots of 2-year-old flower-rose varieties “Black Magic”, “Gold Medal” and “Miss National America” were used as test materials to study the resistance of the stems from April to August by using electrical impedance spectroscopy (EIS) Thermal resistance and the cold resistance from September to January of the following year. The changes of the electrical impedance parameters under high temperature and low temperature stress were studied in detail in order to determine the response of each electrical impedance parameter to temperature stress, The best parameters of semi-lethal temperature and response regression equation. The results show that the curves of extracellular resistivity (re) and relaxation time (τ) with high temperature, re can be fitted well by Logistic equation. The optimal parameters for semi-lethal temperature estimation without temperature treatment are The results were as follows: intracellular resistivity ri (r = 0.795), gold medal ri (r = 0.809) and Miss America (r = -0.858) The parameters of the lethal temperature are respectively “black magic” relaxation time release coefficient ψ (r = 0.967), gold medal re (r = -0.873), Miss America ψ (r = 0.922 ).