论文部分内容阅读
为了比较全面地了解需低温种子休眠和萌发的激素控制机理,我们以室温贮藏为对照,系统研究了低温和外源植物生长调节物对山桃种子萌发的影响,并用气相色谱和气相色谱—质谱联用仪分别测定了内源ABA和IAA在不同贮藏条件下的变化。研究发现:山桃种子去除种皮、子叶后的离体胚轴是不休眠的,在整个室温贮藏期间,种皮对萌发均有显著的抑制作用。去除种皮即可使贮前山桃种子的萌发率由0提高到40％。如果不去除种皮而仅在胚根处划破种皮,可使萌发率提高到26％左右。所以,种皮的抑制作用肯定有一部分是抑制物质引起的。分析证明,种皮ABA含量在贮藏前高达690.07ng/g·FW。干藏16周后仅下降到616.47ng/g·FW。对萌发的抑制作用仍保持原有水平。低温层积6周,种皮ABA含量下降到374.08ng/g·FW,对萌发的抑制作用未见明显减小。到第10周下降为183.74ng/g·FW,种皮对萌发的抑制作用完全解除。去除种皮后,仍有60％的种子不能萌发,而离体胚轴是完全能够迅速萌发的。所以,贮前来自子叶的抑制作用是相当强的。胚的ABA含量在贮前为303.82ng/g·FW,由于子叶比种皮重得多,所以ABA的总含量相当可观。加之子叶与胚之间的直接解剖结构联系,子叶的抑制作用要大于种皮。无论在室温或低温条件下,种子ABA含量均有下降。低温层积6周时,?
In order to understand the hormone control mechanism of dormancy and germination of low-temperature seeds, we systematically studied the effects of low-temperature and exogenous plant growth regulators on the seed germination of peach seeds at room temperature. The effects of gas chromatography and gas chromatography-mass spectrometry The changes of endogenous ABA and IAA under different storage conditions were measured by instrument. The results showed that the seed coat and the cotyledon of hyphae were not dormant, and the seed coat had a significant inhibitory effect on germination during storage at room temperature. The removal of the seed coat can make the germination rate of the seeds of pomegranate increased from 0 to 40%. If you do not remove the seed coat and cut the seed coat only in the radicle, the germination rate can be increased to about 26%. Therefore, the inhibition of the seed coat must be part of the inhibition caused by substances. Analysis showed that seed coat ABA content up to 690.07ng / g · FW before storage. After 16 weeks of storage, it dropped only to 616.47ng / g · FW. Inhibition of germination still maintain the original level. After 6 weeks of cold stratification, the ABA content in the seed coat decreased to 374.08ng / g · FW, and the inhibitory effect on the germination did not decrease obviously. It decreased to 183.74ng / g · FW by the 10th week, and the inhibition of germination by the seed coat completely disappeared. After removing the seed coat, 60% of the seeds are still unable to germinate, while the embryonic axis is completely able to germinate rapidly. Therefore, pre-storage inhibition from cotyledons is quite strong. The ABA content of embryos was 303.82ng / g · FW before storage, and the total content of ABA was considerable because the cotyledons were much heavier than the seed coat. Coupled with the direct anatomy between cotyledons and embryos, cotyledons inhibitory effect is greater than the seed coat. The seed ABA content decreased at either room temperature or low temperature. Cryogenic stratification 6 weeks,?