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目的:建立HPLC法同时测定跌打止痛贴膏中三七皂苷R_1和人参皂苷Rg_1含量的方法。方法:色谱柱为Shim-packCLC-ODS C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.05%磷酸溶液,梯度洗脱;检测波长203 nm,柱温35℃,流速1.0ml·min~(-1)。结果:三七皂苷R_1和人参皂苷Rg_1线性范围分别为0.035 8～0.178 8 mg·ml~(-1)(r=0.999 7),0.225 5～1.128 0mg·ml~(-1)(r=0.999 6);平均回收率分别为96.8%(RSD=2.1%)和97.3%(RSD=2.2%)。结论:本方法简便可靠,重复性好,可用于测定跌打止痛贴膏中三七皂苷R_1和人参皂苷Rg_1的含量。
Objective: To establish a HPLC method for simultaneous determination of notoginsenoside R_1 and ginsenoside Rg_1 in Dacheng Zhitong plaster. Methods: The chromatographic column was Shim-pack CLC-ODS C 18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile-0.05% phosphoric acid solution and gradient elution. The detection wavelength was set at 203 nm and the column temperature was 35 ℃. 1.0ml · min ~ (-1). Results: The linear ranges of notoginsenoside R 1 and ginsenoside Rg 1 were 0.035 8-0.1788 mg · ml -1 (r = 0.999 7), 0.225 5-1.1280 mg · ml -1 (r = 0.999 6). The average recoveries were 96.8% (RSD = 2.1%) and 97.3% (RSD = 2.2%), respectively. Conclusion: The method is simple, reliable and reproducible. It can be used to determine the content of notoginsenoside R_1 and ginsenoside Rg_1.