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目的：探讨呼吸道常见病毒与分泌性中耳炎的关系。方法：应用多聚酶链反应（PCR）检测31例分泌性中耳炎患者的中耳渗出液中腺病毒、衣原体、巨细胞病毒、支原体和疱疹病毒。结果腺病毒阳性者为7／13、衣原体阳性者为5／13、巨细胞病毒阳性者为7／18，未检测出支原体和疱疹病毒。发病期大于14天与小于14天者病原体阳性率有显著性差异，患感冒与无感冒的阳性率间无显著性差异。结论：1）分泌性中耳炎可能与病毒等感染有关；2）早期进行病毒检测对病因的诊断具有重要意义；3）用PCR技术检测中耳渗出液中病毒特异性高，敏感性强。
Objective: To investigate the relationship between common respiratory viruses and secretory otitis media. Methods: The adenovirus, chlamydia, cytomegalovirus, mycoplasma and herpes virus in the middle ear exudates of 31 patients with secretory otitis media were detected by polymerase chain reaction (PCR). Results Adenovirus positive for 7/13, chlamydia positive for 5/13, cytomegalovirus positive for 7/18, no detection of mycoplasma and herpes virus. Incidence of more than 14 days and less than 14 days were significant differences in the positive rate of pathogens, the risk of cold and no positive rate between no significant difference. Conclusions: 1) Secretory otitis media may be related to infection such as virus; 2) early detection of virus is of great significance for the diagnosis of etiology; 3) PCR detection of middle ear exudates is highly specific and sensitive.