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目的对肝癌核心家系进行调查并对其进行分析。建立肝癌核心家系标本数据库,为肝癌遗传易感性的研究提供实验标本和研究资料。方法对经县级及以上医院确诊的肝癌先证者及其血缘父母进行调查和填写调查表,并采集外周静脉血液8ml,其中抗凝血5ml分离、冻存,3ml非抗凝血进行乙肝两对半、AFP及肝功能检测。结果共收集肝癌核心家系251例,具备完整的血样标本及相关资料。调查发现30例家系有肝癌家族史,通过追踪随访新发现肝癌1例。结论建立规范的肝癌核心家系数据库是进行肝癌遗传学研究的基本手段,完善的血样标本及相关资料为后续研究提供了保证,肝癌核心家系标本数据库建立过程中面临的伦理、社会等问题值得探讨。
Objective To investigate and analyze the nuclear family of hepatocellular carcinoma. To establish a database of hepatocellular carcinoma core family specimens to provide experimental specimens and research materials for the study of genetic susceptibility to hepatocellular carcinoma. Methods To investigate and fill in the questionnaire of liver cancer probands and their relatives confirmed by the county level and above hospitals and to collect 8ml of peripheral venous blood, in which 5ml of anticoagulant blood was isolated, frozen and stored in 3ml non-anticoagulant for Hepatitis B Half, AFP and liver function tests. Results A total of 251 nuclear family members of liver cancer were collected, with complete blood samples and related data. The survey found that 30 cases of family history of liver cancer, followed up by follow-up of new cases of liver cancer in 1 case. Conclusion The establishment of a standardized nuclear family database of hepatocellular carcinoma is the basic means to carry out genetic research of hepatocellular carcinoma. The improved blood sample and related data provide a guarantee for follow-up study. The ethical and social problems in establishing the database of hepatocellular carcinoma nuclear family are worth discussing.