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用成年雄性Ｗｉｓｔａｒ大鼠３６只，分为实验组和对照组，对照组动物不做任何处理，实验组动物腹腔注射无菌松节油造成急性非特异性腹膜炎。用免疫组织化学、图像分析和形态计量方法研究了大鼠实验性腹膜炎自愈过程中垂体远侧部ＡＣＴＨ细胞和ＴＳＨ细胞的变化。结果表明，注射后第１ｄ和第３ｄ，ＡＣＴＨ免疫反应细胞的面数密度增大，免疫反应增强（Ｐ＜０．０１）；ＴＳＨ免疫反应细胞面数密度无变化，但在注射后的第３ｄ，ＴＳＨ细胞免疫反应减弱（Ｐ＜０．０５）。第５ｄ，ＡＣＴＨ细胞免疫反应稍有增强、面数密度稍有增大，但同对照组相比无显著性差异（Ｐ＜０．０５）。注射后第７ｄ，ＴＳＨ细胞免疫反应增强（Ｐ＜０．０５）。注射后第１０ｄ，ＡＣＴＨ细胞和ＴＳＨ细胞的面数密度和免疫反应均恢复正常。本研究结果提示实验性腹膜炎自愈过程中，ＡＣＴＨ细胞功能增强而ＴＳＨ细胞功能被抑制并参与了炎症自愈的调节。
Thirty-six adult male Wistar rats were divided into experimental group and control group. The animals in the control group were treated without any treatment. The animals in experimental group were injected with sterile turpentine to cause acute nonspecific peritonitis. The changes of ACTH cells and TSH cells in distal pituitary of rats during experimental peritonitis were studied by immunohistochemistry, image analysis and morphometry. The results showed that on day 1 and day 3 after injection, the number density of ACTH immunoreactive cells increased, and the immune response increased (P <0.01). The number density of TSH immunoreactive cells did not change, , TSH cellular immune response weakened (P <0.05). On the 5th day, the immune response of ACTH cells increased slightly and the number density increased slightly, but there was no significant difference (P <0.05) compared with the control group. On the 7th day after injection, TSH cell immune response was enhanced (P <0.05). On the 10th day after injection, the number density and immune response of ACTH cells and TSH cells returned to normal. The results suggest that during the process of experimental peritonitis self-healing, the function of ACTH cells is enhanced while the function of TSH cells is inhibited and involved in the regulation of inflammatory self-healing.