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近交系C_(57)BL小鼠分别饮用发酵奶、牛奶和自来水。7天后给各组小鼠腹腔注射二甲基肼。24h后处死动物,取大肠固定,按卷帘法石蜡包埋,切片油镜观察20个纵切完整的隐窝,计数上皮细胞中出现微核和凋谢率。经统计学处理,发酵奶组的微核和凋谢率明显低于饮水和饮牛奶组。结果提示Shahani菌株发酵奶能明显抑制二甲基肼对小鼠大肠隐窝上皮细胞的诱变作用。
Inbred C_ (57) BL mice were fed fermented milk, milk and tap water, respectively. Seven days later, mice in each group were injected intraperitoneally with dimethylhydrazine. The animals were sacrificed 24h later, and the colon was fixed. The wounds were embedded in paraffin according to a roller shutter method. Twenty sections of complete slits were observed with a sliced oil microscope, and micronuclei and withering rate were counted in the epithelial cells. After statistical analysis, fermented milk group micronucleus and withered rate was significantly lower than drinking and drinking milk group. The results suggested that the fermentation of Shahani strain could obviously inhibit the mutagenic effect of dimethylhydrazine on the intestinal crypt epithelial cells in mice.