论文部分内容阅读
目的:研究黄芩胶囊法对痤疮的治疗作用,并探讨其作用机制。方法:建立皮脂腺斑痤疮小鼠模型后,给予不同剂量的黄芩胶囊(14、7、3.5 g/kg),连续30d,于实验第34天,采用股动脉采血,分离血清,用放射免疫法检测小鼠血清中T和E2的含量,用ELISA法测定IL-1β、IL-6的含量。处死动物后,用电子游标卡尺测量右侧斑块的最大横径和最大纵径。测量腹腔毛细血管通透性及小鼠耳肿胀程度。并立即取下小鼠侧背部皮脂腺斑组织,10%甲醛固定,石蜡包埋、切片、HE染色,于光镜下观察皮脂腺斑显微结构的变化。结果:与模型组相比,黄芩胶囊14g/kg及安体舒通组6.25g/kg皮脂腺斑较治疗前明显缩小,皮脂腺薄,排列较松散,液化囊形成数目多。与模型组比较,黄芩胶囊各组T、IL-1β、IL-6含量明显降低,E2明显升高。结论:黄芩胶囊能使皮脂腺斑明显缩小,影响皮脂腺增生,抑制皮脂腺细胞的增生及皮脂分泌,其作用机制可能与降低T、IL-1β、IL-6含量,提高E2含量有关。
Objective: To study the therapeutic effect of baicalin capsule on acne and to explore its mechanism. Methods: After establishing the sebaceous acne model mice, different doses of Scutellariae capsules (14, 7, 3.5 g / kg) were given for 30 days. On the 34th day of the experiment, blood was collected from the femoral artery and the serum was separated and detected by radioimmunoassay The contents of T and E2 in the serum of mice were determined by ELISA. The levels of IL-1β and IL-6 were determined by ELISA. After the animals were sacrificed, the maximum diameter and the maximum diameter of the right plaque were measured with an electronic vernier caliper. Peritoneal capillary permeability and mouse ear swelling were measured. The sebaceous gland plaque in the dorsal side of the mice was immediately removed, fixed in 10% formalin, embedded in paraffin, sectioned and stained with HE. The microstructure of sebaceous glands was observed under light microscope. Results: Compared with the model group, the FSH of 14g / kg and 6.25g / kg of spironolactone group were significantly smaller than those before treatment, the sebaceous gland was thin and arranged loosely, and the number of liquefied cysts was more. Compared with the model group, the contents of T, IL-1β and IL-6 in each group were significantly decreased and E2 significantly increased. Conclusion: Scutellaria capsules can significantly reduce the sebaceous glands, affect the proliferation of sebaceous glands and inhibit the proliferation and sebum secretion of sebaceous glands. The mechanism may be related to the decrease of T, IL-1β, IL-6 and the increase of E2 content.