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目的：研究不同性质的羊水注入兔血循环后血中花生四烯酸的代谢产物血栓素Ａ２（ＴＸＡ２）、前列环素Ｉ２（ＰＧＩ２）和白三烯Ｃ４（ＬＴＣ４）的变化。方法：新西兰孕兔２９只，随机分为４组：兔原羊水、胎粪液、胎盘组织提取液和生理盐水，分别在注入前、注入后５ｍｉｎ，、４５ｍｉｎ从兔耳动脉抽血２ｍｌ，用放射免疫分析（ＲＩＡ）检测血中ＴＸＡ２和ＰＧＩ２的代谢物６－酮前列腺素（６－Ｋｅｔｏ－ＰＧＦ１α）和血栓素Ｂ２（ＴＸＢ２），用酶联免疫法（ＥＩＡ）检测ＬＴＣ４。结果：注入胎粪液、胎盘组织提取液后６－Ｋｅｔｏ－ＰＧＦ１α明显增加（Ｐ＜０．０１），兔原羊水组轻度增加，但差异无显著性（Ｐ＞０．０５）；生理盐水组无明显变化（Ｐ＞０．０５）；除生理盐水组外，其余各组的ＴＸＢ２和ＬＴＣ４在注入后增加显著（Ｐ＜０．０１）；ＴＸＢ２、６－Ｋｅｔｏ－ＰＧＦ１α和ＬＴＣ４的组间对比以胎盘组织提取液组增加显著（Ｐ＜０．０１）。结论：羊水进入兔血循环能刺激机体产生ＴＸＡ２、ＬＴＣ４和ＰＧＩ２等花生四烯酸的代谢产物，在羊水栓塞的发生、发展过程中起重要作用。
OBJECTIVE: To study the changes of TXA2, PGI2 and LTC4 in arachidonic acid after the injection of different amniotic fluid into rabbits blood. Methods: A total of 29 New Zealand pregnant rabbits were randomly divided into 4 groups: rabbit amniotic fluid, fetal solution, placental tissue extract and normal saline. Blood samples were taken from the rabbit ear artery 2 min after injection for 5 min, Radioimmunoassay (RIA) was used to detect 6-Keto-PGF1α and TXB2, metabolites of TXA2 and PGI2 in blood. LTC4 was detected by enzyme-linked immunosorbent assay (EIA). Results: 6-Keto-PGF1α was significantly increased (P <0.01) after injection of meconium liquid and placental tissue extract, while the original amniotic fluid of rabbits increased slightly, but the difference was not significant (P> 0.05) (P <0.01). The levels of TXB2, 6-Keto-PGF1α and LTC4 in the other groups increased significantly after injection (P <0.01) Compared with placental tissue extract group increased significantly (P <0.01). CONCLUSION: Amniotic fluid entering the rabbit’s blood circulation can stimulate the production of arachidonic acid metabolites such as TXA2, LTC4 and PGI2 in the body and play an important role in the occurrence and development of amniotic fluid embolism.