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目的观察三氧化二砷(As2O3)大椎穴注射对哮喘大鼠肺组织EOS、血清和支气管肺泡灌洗液(BALF)中IL-17、IL-4的影响。方法将大鼠随机分为对照组、哮喘组、As2O3组和地塞米松(Dx)组,每组10只。采用卵白蛋白复制大鼠哮喘模型,并给予各组相应的处理。检测各组大鼠支气管黏膜下层EOS数及气道壁EOS凋亡指数,血清和BALF中IL-4、IL-17的含量变化。结果①支气管黏膜下EOS数:哮喘组高于对照组(P<0.01);As2O3组和Dx组低于哮喘组(P<0.01),但高于对照组(P<0.01);两治疗组之间比较,差异无统计学意义(P>0.05)。②EOS凋亡指数:哮喘组明显低于对照组(P<0.01);As2O3组多于哮喘组但低于对照组,差异均有统计学意义(P<0.01);Dx组明显高于哮喘组(P<0.01)。③哮喘组BLAF中IL-4、IL-17含量明显高于对照组(P<0.01),As2O3组和Dx组血清IL-4、IL-17含量低于哮喘组(P<0.01),高于对照组(P<0.01),但两治疗组之间差异无统计学意义(P>0.05)。结论三氧化二砷大椎穴注射能抑制哮喘大鼠气道炎症。
Objective To observe the effect of Dazhui (As2O3) injection on the levels of IL-17 and IL-4 in lung tissue of asthmatic rats with EOS, serum and bronchoalveolar lavage fluid (BALF). Methods The rats were randomly divided into control group, asthma group, As2O3 group and dexamethasone group (n = 10). Ovalbumin was used to replicate the rat asthma model and the groups were treated accordingly. The number of EOS in bronchus submucosa, the index of EOS in airway wall and the levels of IL-4 and IL-17 in serum and BALF of rats in each group were detected. Results (1) The number of bronchial submucosal EOS in asthma group was higher than that in control group (P <0.01), while that in As2O3 group and Dx group was lower than that in asthma group (P <0.01), but higher than that in control group (P <0.01) Between the two groups, the difference was not statistically significant (P> 0.05). (2) The apoptotic index of EOS in asthma group was significantly lower than that in control group (P <0.01); As2O3 group was more than asthma group but lower than that in control group (P <0.01); Dx group was significantly higher than that in asthma group P <0.01). ③ The levels of IL-4 and IL-17 in BLAF of asthma group were significantly higher than those in control group (P <0.01), while the levels of IL-4 and IL-17 in As2O3 group and Dx group were lower than those in asthma group (P <0.01) Control group (P <0.01), but there was no significant difference between the two treatment groups (P> 0.05). Conclusion Arsenic Trioxide Dazhui acupoint can inhibit airway inflammation in asthmatic rats.