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钙振荡(calcium oscillation) 能以频率解码的形式调节基因转录,钙振荡的频率可反应基因转录的水平. 为探索持续缺氧是强化还是钝化肺动脉内皮细胞对组胺的反应,研究了24 h 亚急性轻度缺氧对组胺刺激的肺动脉内皮细胞钙振荡频率的影响,并探索了其机制. 结果是:a. 24 h 亚急性轻度缺氧可显著增加组胺刺激的肺动脉内皮细胞钙振荡频率;b.N A D PH 氧化酶抑制剂,diphenylene iodonium chloride (D PI,10 μm ol/L) 消除了组胺刺激的常氧和缺氧后肺动脉内皮细胞钙振荡;c. 黄嘌呤氧化酶抑制剂,别嘌呤醇(oxypurinol,100 μm ol/L) 能显著降低组胺刺激的缺氧后肺动脉内皮细胞升高的钙振荡频率,但降低后的钙振荡频率仍高于常氧组,别嘌呤醇对组胺刺激的常氧组肺动脉内皮细胞钙振荡频率无显著影响. 以上结果表明,在持续缺氧相关的肺疾患中,肺动脉内皮细胞对组胺反应的敏感性增加. N A D PH 氧化酶在组胺刺激的钙振荡的发生中发挥重要作用;黄嘌呤氧化酶的激活是缺氧引起组胺刺激的钙振荡频率增加的重要原因.
Calcium oscillation regulates gene transcription in the form of frequency decoding, and the frequency of calcium oscillation reflects the level of gene transcription.In order to explore whether persistent hypoxia enhances or inactivates pulmonary arterial endothelial cells response to histamine, the effects of 24 h The effects of subacute mild hypoxia on calcium oscillation frequency of pulmonary arterial endothelial cells stimulated by histamine were explored and the mechanism was explored.The results were as follows: a. 24 h subacute mild hypoxia could significantly increase histamine-stimulated pulmonary artery endothelial calcium Oscillation frequency; bN AD PH oxidase inhibitor, diphenylene iodonium chloride (D PI, 10 μmol / L) abolished histamine-induced normoxia and hypoxia-induced calcium oscillations in pulmonary artery endothelial cells; c. Xanthine oxidase inhibitor , Oxypurinol (100 μmol / L) significantly decreased the frequency of calcium oscillations induced by histamine-stimulated pulmonary artery endothelial cells after hypoxia, but the decreased calcium oscillation frequency was still higher than that of normoxia group and allopurinol There was no significant difference in the frequency of calcium oscillations in normoxic pulmonary artery endothelial cells stimulated by histamine.The above results indicate that the sensitivity of pulmonary arterial endothelial cells to histamine response is increased in the persistent hypoxia-related lung diseases. D PH oxidase plays an important role in the histamine-stimulated calcium oscillation. The activation of xanthine oxidase is an important reason for the increase of histamine-stimulated calcium oscillation frequency induced by hypoxia.