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目的:探讨哌立福辛对体外培养的人胃癌SGC7901细胞生长增殖的影响并探讨其机制。方法:分别用不同浓度的哌立福辛(0.125、0.250、0.500、0.750、1.500μmol/L)对SGC7901细胞进行干预后,采用磺酰罗丹明B(SRB)法检测细胞增殖变化;平板克隆实验检测细胞克隆形成能力;Real-time PCR、Western blot法检测癌症相关基因eIF4E及AEG-1 mRNA及蛋白表达。结果:哌立福辛呈时间及浓度依赖性抑制胃癌SGC7901细胞的增殖,并降低细胞的克隆形成能力;细胞内AKT信号通路中AEG-1、eIF4E的表达明显降低。结论:哌立福辛具有抑制胃癌SGC7901细胞增殖的作用;通过降低AKT信号通路中AEG-1和eIF4E的mRNA及蛋白表达可能是发挥增殖抑制作用的机制之一。
Objective: To investigate the effect of perifoxine on the growth and proliferation of human gastric cancer cell line SGC7901 cultured in vitro and its mechanism. Methods: SGC7901 cells were treated with different concentrations of perifosine (0.125,0.250,0.500,0.750,1.500μmol / L) respectively, and the proliferation of SGC7901 cells was detected by SRB method. The results of plate clone assay Real-time PCR and Western blot were used to detect the expression of eIF4E and AEG-1 mRNA and protein. Results: Periflocin inhibited the proliferation of SGC7901 cells in a time and concentration-dependent manner and decreased the clonality of cells. The expression of AEG-1 and eIF4E in the AKT signaling pathway was significantly decreased. CONCLUSION: Perifosine inhibits the proliferation of gastric cancer cell line SGC7901. It may be one of the mechanisms that inhibit the proliferation of SGC7901 cell line by decreasing the mRNA and protein expression of AEG-1 and eIF4E in AKT signaling pathway.