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目的:研究环瓜氨酸肽(CCP)辅助完全弗氏佐剂(CFA)造模对佐剂性关节炎模型大鼠临床体征、血清因子、病理的影响。方法:SD大鼠随机分为4组,即空白对照组、完全弗氏佐剂雄性组(雄CFA组)、完全弗氏佐剂雌性组(雌CFA组)、完全弗氏佐剂+环瓜氨酸肽组(CCP+CFA组)。除空白对照组注射0.1ml生理盐水外,雄CFA组和雌CFA组注射0.1ml CFA,CCP+CFA组注射含有100μg环瓜氨酸肽的0.1ml CFA,造模后每隔3d观察大鼠足肿胀度,第28d处死取血清ELISA法检测IL-6、TNF-a、RF、anti-CCP,致炎侧踝关节进行病理检测。结果:与空白对照组比较,雄CFA组、雌CFA组和CCP+CFA组大鼠关节肿胀度在各个时间段均显著增大,但各模型组之间无显著的统计学意义;与空白对照组比较,各模型组IL-6、TNF-a、RF、anti-CCP均明显升高,具有极显著的统计学意义,与雄CFA组比较,CCP+CFA组IL-6、TNF-a、anti-CCP含量均升高,具有显著的统计学意义,但雌、雄CFA组之间无显著差异;与空白组比较,各模型组炎性细胞浸润、滑膜组织增生、纤维组织增生、巨噬细胞增生、骨质侵蚀水平均明显升高,具有显著的统计学意义,但各模型组之间无统计学意义。结论:环瓜氨酸肽作为一种类风湿关节炎造模的辅助剂,其血清IL-6、TNF-a、RF、anti-CCP均较普通模型显著,可以应用到类风湿关节炎的研究中。
Objective: To investigate the effects of cyclic citrullinated peptide (CCP) adjuvant complete Freund’s adjuvant (CFA) on clinical signs, serum factors and pathology in rats with adjuvant arthritis. Methods: SD rats were randomly divided into 4 groups: blank control group, complete Freund’s adjuvant male group (male CFA group), complete Freund’s adjuvant female group (female CFA group), complete Freund’s adjuvant + Amino peptide group (CCP + CFA group). In addition to the blank control group injected with 0.1ml saline, the male CFA group and the female CFA group injected 0.1ml CFA, CCP + CFA group injected with 0.1μg CFU containing 100μg of cyclic citrullinated peptide, modeling observed every three days rat foot Twenty-eight days later, serum IL-6, TNF-α, RF, anti-CCP and inflammatory side ankle joint were detected by ELISA. Results: Compared with the blank control group, the degree of swelling of joints in the male CFA group, the female CFA group and the CCP + CFA group increased significantly at all time points, but there was no significant difference between the model groups and the blank control group The levels of IL-6, TNF-a, RF and anti-CCP in each model group were significantly higher than those in the control group anti-CCP content were increased, with significant statistical significance, but there was no significant difference between the female and male CFA groups; Compared with the blank group, inflammatory cell infiltration, synovial tissue hyperplasia, fibrous tissue hyperplasia, giant Phagocytic proliferation, bone erosion were significantly increased, with significant statistical significance, but no significant difference between the model groups. CONCLUSION: As a rheumatoid arthritis model adjuvant, cyclic citrullinated peptide has significantly higher serum IL-6, TNF-a, RF and anti-CCP than normal model and can be used in the research of rheumatoid arthritis .