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目的本文旨在研究长期感觉剥夺后,背缝神经核的电刺激对桶状皮层(barrel cortex)IV层神经元的神经应答特性的影响。方法雄性Wistar大鼠分为感觉剥夺组(sensory-deprived,SD)和对照组。SD组大鼠从出生开始,左半脸所有胡须(D2胡须除外)均被拔除,并维持60天。在之后的8-10d内,允许胡须重新生长。对背缝神经核给予电刺激,之后偏转胡须,记录D2桶状皮层IV层神经元的神经应答。结果当给予的电刺激的间距为50 ms和100 ms时,电刺激能增强SD大鼠主须(principal whisker,PW)偏转引起的神经应答,而当给予的电刺激的间距为800 ms时,电刺激能增强SD组和对照组大鼠邻近胡须(adjacent whisker,AW)偏转引起的神经应答。与对照组相比,胡须的拔除能增强桶状皮层对PW偏转的应答,而削弱其对AW偏转的应答。此外,PW或AW偏转后,在两组中均未发现与背缝神经核的电刺激相关的反应潜伏期的变化。与对照组相比,SD大鼠的桶状皮层中5-HT2A受体的表达水平无明显差异。结论背缝神经核的电刺激能调节感觉剥夺的桶状皮层的信息处理。
Objective This study aimed to investigate the effect of electrical stimulation of dorsal raphe nucleus on the neurological response of barrel cortex neurons after long-term sensory deprivation. Methods Male Wistar rats were divided into sensory deprived (SD) group and control group. SD rats were born from birth, all the beard left face (except for D2 beard) were removed and maintained for 60 days. In the next 8-10days, the beard is allowed to regrow. Electric stimulation of the dorsal raphe nucleus was given, after which the beard was deflected to record the neural response of neurons in layer D2 of the barrel of the barrel. Results When the electrical stimulation was given at a distance of 50 ms and 100 ms, electrical stimulation enhanced the neurological response induced by the deflection of the principal whisker (PW) in SD rats. When the electrical stimulation was given at a distance of 800 ms, Electrical stimulation enhanced the neural response induced by the adjacent whisker (AW) deflection in SD and control rats. Compared with the control group, the removal of beard enhances the response of the barrel cortex to PW deflection, which diminishes its response to AW deflection. In addition, no changes in response latency associated with electrical stimulation of dorsal raphe nucleus were found in either group after PW or AW deflection. Compared with the control group, there was no significant difference in the expression level of 5-HT2A receptor in the barrel cortex of SD rats. Conclusion The electrical stimulation of the dorsal raphe nucleus regulates the sensory deprivation of the barrel cortex.