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目的探讨女性HPV DNA检测在宫颈癌防治方面的意义。方法应用DNA杂交技术对518例妇科门诊就诊者基因分型检测。结果518例样本中,HPV感染有267例,阳性率51.54%。检测高危型HPV(16,18,31,33,35,39,45,51,52,56,58,59,68)218例,占感染病例为81.65%;检出低危型HPV(6,11,42,43,44)42例,占感染病例为15.73%;中国人群常见型HPV(53,66,CP8304)7例,占感染率为2.62%。86例样本中包含了2-5种亚型的感染。结论DNA杂交技术检测HPV基因分型,可1次检测多种亚型,有利于对HPV多重感染的诊断和宫颈癌的防治,可作为宫颈癌筛查的手段。
Objective To investigate the significance of detecting HPV DNA in cervical cancer prevention and treatment. Methods The genotypes of 518 gynecological outpatients were detected by DNA hybridization. Results Among 518 samples, HPV infection was found in 267 cases, the positive rate was 51.54%. 218 cases of high-risk HPV (16,18,31,33,35,39,45,51,52,56,58,59,68) were detected, accounting for 81.65% of all the cases. Low-risk HPV (6, 42 cases, accounting for 15.73% of cases of infection; 7 cases of Chinese common type of HPV (53,66, CP8304), accounting for 2.62%. Eighty-five samples contained infections of 2-5 subtypes. Conclusion DNA hybridization can detect HPV genotypes and detect multiple subtypes at one time, which is helpful for the diagnosis of multiple HPV infection and prevention and treatment of cervical cancer. It can be used as a screening tool for cervical cancer.