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Due to increasingly stringent environmental pollution norms,there is a need for alteate combustion techniques and alteate fuels to keep up with changing trends.One of the viable solutions for India is the adaptation of methanol as a fuel for automotive sector.Therefore,in this study a functional two-wheeler prototype was developed,which uses M85 (85％ v/v methanol and 15％ v/v gasoline) in an electronic control unit (ECU) controlled port fuel injected (PFI) engine.This study included comparative investigations of simulated on-road two-wheeler performance on chassis dynamometer using a gasoline-fuelled motorcycle with stock ECU vis-à-vis M85-fuelled motorcycle using recalibrated ECU.ECU recalibration exhibited that M85-fuelled vehicle was operated at relatively more advanced spark timing compared to baseline gasoline-fuelled vehicle.Performance results showed that M85-fuelled motorcycle produced relatively higher engine power and higher maximum vehicle speed compared to gasoline-fuelled motorcycle.Relatively superior acceleration characteristics (especially at higher speeds) of M85-fuelled motorcycle was another important finding of this study,indicating that M85 provided superior throttle response compared to gasoline.Comparative analysis of raw tailpipe emissions showed that modified M85-fuelled motorcycle emitted relatively higher hydrocarbon (HC),carbon monoxide (CO) and oxides of nitrogen (NOx) emissions compared to stock gasoline-fuelled motorcycle.However,these emissions can be controlled by using adaptation of suitable after-treatment systems.