论文部分内容阅读
本工作采用渐进性低氧法降低总氧运输量，从而观察抗重组人肿瘤坏死因子（rhTNFα）单克隆抗体对内毒素血症家兔氧提取功能的影响。结果表明，在DO2－VO2的关系中，抗体保护组类同于对照组，可清楚地分为“非依赖”与“依赖”两部分；无关抗体组类同于内毒素组，从呼吸空气开始从未出现坪区。抗体保护组的临界氧运输量（DO2c）和合并氧提取率分别为10．80±3．21ml／（min·kg）和0．690，与对照组（分别为10．18±1．69和0．730）无显著差异（P＞0．05）；无关抗体组合并氧提取率为0．408，显著低于抗体保护组（P＜0.05）。在血浆中TNFα浓度，无关抗体组在各时间点上均显著高于抗体保护组（P＜0.01）。实验结果表明，精确特异性的重组人TNFα单抗具有阻断或逆转内毒素血症氧提取障碍的病理过程。
In this work, we used progressive hypoxia to reduce the total oxygen transport, so as to observe the effect of anti-recombinant human tumor necrosis factor (rhTNFα) monoclonal antibody on the oxygen extraction of endotoxemia rabbits. The results showed that in the relationship of DO2-VO2, the antibody protection group was similar to the control group, which could be clearly divided into two parts: “independent” and “dependent”; unrelated antibody group was similar to endotoxin group, From the breathing air never appeared Ping area. The critical oxygen delivery (DO2c) and combined oxygen extraction rates of the antibody protection group were 10.80 ± 3.21 ml / (min · kg) and 0.690, respectively, which were significantly lower than those of the control group (10.18 ± 1.69 and 0.730) (P> 0.05). The oxygen extraction rate of unrelated antibody combinations was 0.408, which was significantly lower than that of antibody protection group (P <0.05). In the plasma TNFα concentration, irrelevant antibody group was significantly higher than the antibody protection group at each time point (P <0.01). The experimental results show that the precise and specific recombinant human TNFα monoclonal antibody has the pathological process of blocking or reversing the oxygen extraction obstacle of endotoxemia.