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Using continuous cultures of ECC_2/HNstrain of P.falciparum,we found that Qing-haosu(QHS)at a concentration of 1×10~(-8)M hadno appreciable effect on the growth of the para-sites,while QHS at 1×10~(-8)M markedly inhibit-ed their growth and multiplication.This indi-cated the possibility of QHS exerting direct ef-fect on the erythrocytic stage of P.falciparumin vitro.Electron microscopy showed morpho-logical abnormalities appearing in the asexualforms of P.falciparum following exposure toQHS at 1×10~(-7)M or 5×10~(-7)M for 2 hours or 1hour respectively.The main pathological chan-ges were:(1)injuries to the membrane struc-ture,such as widening of the intermembranousspace between the nuclear membrane and thelimiting membrane,swollen endoplasmic re-ticulum,etc.;(2)formation of autophagic va-cuoles containing no pigment.For comparisonwith QHS,the action of CQ on the ultrastruc-ture of the parasite was also investigated anddiscussed.
Using continuous cultures of ECC_2 / HNstrain of P. falciparum, we found that Qing-haosu (QHS) at a concentration of 1 × 10 -8 M hadno appreciable effect on the growth of the para-sites, while QHS at 1 × 10 ~ (-8) M markedly inhibit-ed their growth and multiplication. This indi-cated the possibility of QHS exerting direct ef-fect on the erythrocytic stage of P. falciparum in vitro. Electron microscopy showed morpho-logical abnormalities appearing in the asexualforms of P. falciparum following exposure to QHS at 1 × 10 -7 M or 5 × 10 -7 M for 2 hours or 1 hour respectively. The main pathological chan-ges were: (1) injuries to the membrane struc-ture, such as widening of the intermembranous space between the nuclear membrane and the limiting membrane, swollen endoplasmic re-ticulum, etc .; (2) formation of autophagic va-cuoles containing no pigment. For comparison with QHS, the action of CQ on the ultrastruc-ture of the parasite was also investigated and didiscussed.