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In this paper, the characteristics of separating xylitol, arabinitol and sorbitol with uniformly loaded columns containing 2,5,7 and 9wt% XE-60 and gradient loaded columns are compared in detail. The suspended chain curve formulaY=en (x-a/2) +e-n (x-a/2) is suggested for calculation of amount of different coated packing in the gradient loaded column, where selector coefficient n=l.l, a is average liquid loading amount, x is individual liquid loading amount. The gradient loaded column can not only satisfactorily separate isomers of xylitol and arabinitol, but also shorten retention time of sorbitol obviously.
In this paper, the characteristics of the separating xylitol, arabinitol and sorbitol with uniformly loaded columns containing 2,5,7 and 9 wt% XE-60 and gradient loaded columns are compared in detail. The suspended chain curve formula Y = en (xa / 2) + en (xa / 2) is suggested for calculating of amount of different coated packing in the gradient loaded column, where selector coefficient n = ll, a is average liquid loading amount, x is individual liquid loading amount. only satisfactorily separate isomers of xylitol and arabinitol, but also shorten retention time of sorbitol obviously.