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Summary: The vacuolated effect of Helicobacter (H. pylori) and its relationship to vacuolated cytotoxin antigen (VacA) were investigated by the method of cytotoxic test and SDS-pobyacrylamide gel electrophoresis (SDS-PAGE). Of the 62 clinical isolates, the broth culture filter (BCF) of 43 strains caused the Vero cell intracytoplasmically vacuolated. H. pylori strains were divided into H. pylori (Toxin +) group with vacuolated effect and H. pylori (Toxin -) group without vacuolated effect. The analysis of the BCF of H. pylori (Toxin +) and that of H. pylori (Toxin -) was studied by SDS-PAGE and Scan reader. A kind of protein with 87 ku molecular weight was recognized in the BCF of 30.23 % (13/43) H. pylori (Toxin +) strains but in none of that of H. pylori (Toxin -) strains, the difference was statistically significant (P<0.05). There was a significant and concordant relationship between OD of the protein band with 87 ku molecular weight and titer of vacuolated activity of H. pylori(Toxin +) (r=0.67 and P<0.05 by linear regression analysis). H. pylori strains were divided into H. pylori (Toxin +) group with vacuolated effect and H. pylori (Toxin -) group without vacuolated effect. The vacuolated effect of H. pylori (Toxin +) was caused by the protein with 87 ku molecular weight (VacA)
Summary: The vacuolated effect of Helicobacter (H. pylori) and its relationship to airlated cytotoxin antigen (VacA) were investigated by the method of cytotoxic test and SDS-pobyacrylamide gel electrophoresis (SDS-PAGE). Of the 62 clinical isolates, the broth H. pylori (Toxin -) group with plasmalated effect and H. pylori (Toxin -) group without vacuolated effect. The analysis of the BCF of H. pylori (Toxin +) and that of H. pylori (Toxin -) was studied by SDS-PAGE and Scan reader. A kind of protein with 87 ku molecular weight was recognized in the BCF of 30.23% (13/43 There was a significant and concordant relationship between OD of the protein band with 87 (p <0.05). The H. pylori (Toxin +) strain but in none of that of H. pylori ku molecular weight and titer of the vacuumed activity of H. pyl H. pylori was expressed in H. pylori (Toxin +) group with plasmalated effect and H. pylori (Toxin -) group without vacuolated effect. The vacuolated effect of H. pylori (Toxin +) was caused by the protein with 87 ku molecular weight (VacA)