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目的:前期研究发现自发性高血压大鼠(SHR)14~18周龄表现为稳定肝火亢盛证,现从宏观表征及行为学,微观理化指标,方剂反证验证,初步研究高血压肝火亢盛证生物学机制。方法:14周龄SHR 55只经证候属性判别,筛选出肝火亢盛证SHR,根据收缩压差异用拉丁方分组法将其分为:模型组、三草降压汤组、四逆散组和硝苯地平组,正常血压大鼠(WKY)为正常组。动态采集大鼠宏观表征、行为学指标和收缩压;酶联免疫吸附试验(ELISA)法检测大鼠给药4,6周时血清5-羟色胺(5-HT),血管紧张素Ⅱ(ANGⅡ),去甲肾上腺素(NE)含量。结果:与正常组相比模型组14~20周龄血压值和易激惹程度升高(P<0.05),旋转耐受时间减少,且血清5-HT,ANGⅡ,NE升高。与模型组相比三草降压汤组和硝苯地平组给药4,6周收缩压降低(P<0.05),四逆散组收缩压先升高后降低,不稳定;三草降压汤组14~18周龄肝火亢盛证症状包括旋转耐受时间,易激惹程度和宏观表征有较明显的改善,四逆散组改善不明显,三草降压汤组血清5-HT,ANGⅡ,NE降低。结论:14~18周龄是SHR稳定的肝火亢盛证期,5-HT,ANGⅡ,NE升高可作为高血压肝火亢盛证客观指标。
OBJECTIVE: The previous study found that spontaneous hypertensive rats (SHR) 14 to 18 weeks of age showed a stable liver fire Sheng Sheng card, macroscopic characterization and behavioral studies, micro-physical and chemical indicators, prescription evidence verification, preliminary study of hyperthyroidism Sheng card syndrome biological mechanism . Methods: The 14-week-old SHR 55 was identified by syndrome differentiation and screened SHR. According to the difference of systolic pressure, SHR 55 was divided into three groups: model group, Sancao Jiangcao Decoction, Sini Decoction and Nitrate Phenytoin group, normal blood pressure rats (WKY) for the normal group. The macroscopic characterization, behavioral indexes and systolic pressure of rats were dynamically collected. Serum levels of 5-HT, ANGII and TNF-α were detected by enzyme linked immunosorbent assay (ELISA) , Norepinephrine (NE) content. Results: Compared with the normal group, the blood pressure and irritability of the model group increased from 14 to 20 weeks (P <0.05), the rotation tolerance time decreased, and the serum 5-HT, ANGⅡ, NE increased. Compared with the model group, the systolic blood pressure of Sancao Jiangcui Decoction group and nifedipine group decreased 4 and 6 weeks (P <0.05), and the systolic blood pressure of Sini Decoction group increased first and then decreased and became unstable; Soup group of 14 to 18 weeks of liver hyperthyroidism Symptoms include rotation tolerance time, irritability and macroscopic characterization of a more significant improvement, Sini Powder group did not improve significantly, Sancao Jiang Ya Tang serum 5-HT, ANG Ⅱ, NE decreased. Conclusion: The 14-18 weeks old is the stable period of SHR. The increase of 5-HT, ANGⅡ, NE can be regarded as the objective index of hypertensive liver-fire syndrome.