论文部分内容阅读
目的:探讨茶色素对人肺鳞癌细胞株SK-MES-1细胞生长及凋亡的影响。方法:以超声细胞破碎法提取茶色素并计算得率。常规培养SK-MES-1细胞株,采用噻唑蓝溴化四唑(MTT)比色法观察不同浓度(5、2.5、1.25、0.625、0.3125 mg/m L)茶色素作用24、48 h对SK-MES-1细胞株生长的抑制作用,计算生长抑制率及IC_(50);以流式细胞术(FCM)荧光双染法(Annexin V/PI)观察茶色素对SK-MES-1细胞株凋亡的影响。结果:茶色素提取得率为7.35%。MTT实验结果显示随茶色素浓度增高和培养时间延长,其对细胞的生长抑制率也相应升高,即呈现剂量-时间依赖性,24 h和48 h处理的IC_(50)分别为2.353 mg/m L和1.494 mg/m L。流式细胞术检测作用24 h细胞凋亡,空白对照、0.625 mg/m L和1.25 mg/m L剂量组的SK-MES-1细胞凋亡率分别为7.7%、20.37%和25.25%。结论:茶色素可促进SK-MES-1细胞凋亡并抑制其增殖。
Objective: To investigate the effect of tea pigment on the growth and apoptosis of human lung squamous cell carcinoma cell line SK-MES-1. Methods: Ultrasound cell disruption method to extract tea pigment and calculate the yield. SK-MES-1 cells were cultured routinely. The effect of different concentrations of tea pigments (5, 2.5, 1.25, 0.625, 0.3125 mg / mL) -MES-1 cell line and the growth inhibition rate and IC 50 were calculated. The effect of tea pigment on the SK-MES-1 cell line was observed by flow cytometry (FCM) double staining (Annexin V / PI) Effect of apoptosis. Results: The yield of tea pigment was 7.35%. The results of MTT showed that with the increase of tea pigment concentration and prolongation of culture time, the growth inhibition rate of the cells increased correspondingly, that is dose-time dependent. The IC 50 of 24 h and 48 h treatment were 2.353 mg / m L and 1.494 mg / m L. Flow cytometry showed that the apoptotic rates of SK-MES-1 cells were 7.7%, 20.37% and 25.25% at 24 h, blank control, 0.625 mg / ml and 1.25 mg / ml respectively. Conclusion: Tea pigment can promote SK-MES-1 cell apoptosis and inhibit its proliferation.