论文部分内容阅读
目的对白纹伊蚊雌蚊各个组织器官的Wolbachia和WO噬菌体进行时空定量分析,并从量上寻找Wolbachia、WO噬菌体和宿主三者之间的相关性,为进一步揭示这三者之间相互作用的分子机制奠定基础。方法分别于第1、5、10、15、20、30、40d各取雌蚊10只,麻醉后于解剖镜下解剖蚊虫的头部、卵巢、中肠、马氏管、脂肪体和胸部,各自提取其基因组DNA,以蚊虫基因rpS7为内参,Q-PCR检测各个组织中的Wolbachia和WO噬菌体的在白纹伊蚊雌蚊体内的平均相对含量和时空动态变化,对Wolbachia和WO噬菌体的拷贝数进行量化关系转化。结果 Q-PCR结果显示Wolbachia和WO噬菌体在蚊虫的卵巢中含量最大,其次是脂肪体、马氏管和头部,而在中肠和胸部的含量最小。发现在蚊虫d龄前30d中,wAlbB与WO噬菌体的动态变化趋势基本一致,且从拷贝数量上二者存在着线性关系。结论白纹伊蚊雌蚊的各个组织均存在wAlbA和wAlbB的双重感染和WO噬菌体的侵染,且这三者在卵巢中的含量远大于其他组织部位。WO噬菌体主要侵染的对象可能是wAlbB,而不是wAlbA。
Objective To quantitatively analyze the spatial and temporal distribution of Wolbachia and WO phages in various tissues and organs of Aedes albopictus mosquitoes, and to find out the correlation between Wolbachia, WO phage and host in quantity. In order to further reveal the interaction between the three Molecular mechanisms to lay the foundation. Methods Ten female mosquitoes were collected on the 1st, 5th, 10th, 15th, 20th, 30th and 40th days respectively. The head, ovary, midgut, Their genomic DNAs were extracted and the mosquito gene rpS7 was used as an internal reference. The relative content and spatio-temporal dynamics of Wolbachia and WO phage in each mosquito were determined by Q-PCR. The changes of Wolbachia and WO phage Number of quantitative relationship transformation. Results The results of Q-PCR showed that the Wolbachia and WO phages were the most abundant in the ovaries of the mosquitoes, followed by fat body, Malpighian tube and head, while the contents were the lowest in midgut and chest. The trend of the dynamic changes of wAlbB and WO phages was basically the same 30 days before the age of d mosquitoes, and there was a linear relationship between them. Conclusions The double infection of wAlbA and wAlbB and the infection of WO phage exist in all tissues of Aedes albopictus mosquitoes, and their content in ovary is much higher than that of other tissues. The main target of the WO phage infection is wAlbB, not wAlbA.