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抑制血管生成是肿瘤治疗的重要方法之一,若能从分子水平检测血管损伤的机制,对治疗将更具指导意义。拉曼光谱是一种无损、信息非常丰富的光谱技术,可以应用于固态、溶液或液态的生物分子分析。基于激光共焦拉曼光谱技术设计一方案,测量分析了鸡胚微血管在抗血管药物沙利度胺作用前后的拉曼光谱。加药5 h与对照组血管的平均拉曼谱在位置和形状上类似,但某些特征峰在强度上却发生变化,如波数1441 cm-1,1527 cm-1及1657 cm-1的强度明显增强而971 cm-1和1081 cm-1的强度明显减弱,这些变化可能是药物作用血管后,使血管内的核酸、蛋白质、磷脂等重要生物分子在结构或含量上发生了不同的改变而引起的。研究结果表明拉曼光谱有可能成为从分子水平分析抗血管生成药物对血管作用的一种有效方法。
Inhibition of angiogenesis is an important method of cancer treatment, if we can detect the mechanism of vascular injury from the molecular level, the treatment will be more instructive. Raman spectroscopy is a nondestructive, highly informative spectroscopic technique that can be applied to biomolecular analysis in solid, solution or liquid state. A protocol based on laser confocal Raman spectroscopy was designed to measure and analyze the Raman spectra of chicken embryo microvessels before and after anti-vascular drug thalidomide. The average Raman spectra of the blood vessels in the 5-day dosing group were similar in position and shape to those in the control group, but some of the characteristic peaks varied in intensity, such as wave numbers 1441 cm-1, 1527 cm-1 and 1657 cm-1 Significantly increased while the intensity of 971 cm-1 and 1081 cm-1 decreased significantly. These changes may be caused by the changes in the structure or content of important biological molecules such as nucleic acids, proteins, and phospholipids in blood vessels after the drug acts on the blood vessels caused. The results show that Raman spectroscopy may be an effective method to analyze the effect of antiangiogenic drugs on blood vessels at the molecular level.