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产甲烷菌对环境变化的敏感性很容易导致厌氧发酵失败,如何保证产甲烷菌的活性是厌氧发酵稳定进行的关键。在考察RY3、SH4、G1、G2和G3产甲烷菌株主要生理生化特征和拮抗作用的基础上,构建了产甲烷复合菌剂,并对产甲烷复合菌剂的pH值耐受性、温度耐受性和不同接种量进行了性能评价。结果表明:5株互补共生构建的产甲烷复合菌剂可在pH值5.5~10.5的范围内生长,且在pH值5.5~9.5的范围内培养3 d后甲烷总产量在1 706.7~2 026.7μmol之间,具有较优良的耐酸碱性能;产甲烷复合菌剂的生长温度范围在15~70℃,且在30~55℃范围内培养3d后甲烷总产量在1 906.9~2 028μmol之间,温度适应范围宽泛。产甲烷复合菌剂接种量试验表明,在低温20℃下,接种产甲烷合菌剂的试验组比未接种复合菌剂对照组在产甲烷的时间上平均缩短14 d,在高温50℃下,接种产甲烷复合菌剂的试验组比未接种产甲烷复合菌剂的对照组在产甲烷的时间上平均缩短5 d,无论低温还是高温下,复合菌剂的接种均可明显促进产甲烷过程的启动,缩短启动时间。中试产气效果及动力学分析表明,20℃低温下,接种10%复合菌剂的试验组21 d内沼气总产量和甲烷总产量均为接种10%活性污泥试验组的1.6倍;50℃高温下,接种10%复合菌剂的试验组21 d内沼气总产量为接种10%活性污泥试验组的2.7倍,甲烷总产量为2.8倍,无论低温20℃还是高温50℃下,接种复合菌剂的可显著提高厌氧发酵产沼气效率,缩短产甲烷进程,为厌氧发酵系统优化调控提供一种新的技术途径。
Methanogenic bacteria sensitivity to environmental changes can easily lead to anaerobic fermentation failure, how to ensure methanogenic activity is the key to the stability of anaerobic fermentation. Based on the investigation of the main physiological and biochemical characteristics and antagonism of methanogenic strains RY3, SH4, G1, G2 and G3, the methane-producing compound fungicides were constructed and their pH tolerance, temperature tolerance Sexual and inoculation performance was evaluated. The results showed that the five methanogenic compounds could grow within the pH range of 5.5-10.5 and the total methane production ranged from 1 706.7 to 2 026.7 μmol after 3 d culture at pH 5.5-9.5 , And had better acid and alkali resistance. The growth temperature of methanogenic composite bacterium was in the range of 15 ~ 70 ℃, and the total methane output was in the range of 1 906.9 ~ 2082μmol after cultured in the range of 30 ~ 55 ℃ for 3d, Wide range of temperature adaptation. Inoculation experiments showed that in the low temperature of 20 ℃, inoculation of methanogenic agents in the test group than inoculated with compound bacteria agent control group in methane production time shortened by 14 days on average, at a high temperature of 50 ℃, Compared with the control group inoculated with methane-producing compound fungicides, the experimental group inoculated with methanogenic composite fungi shortened the methane production time on average by 5 days. The inoculation of compound inoculant obviously promoted the methane-producing process at both low temperature and high temperature Start, shorten the start-up time. Pilot gas production and kinetic analysis showed that in the 20 ℃ low temperature, the total biogas production and total methane production in the test group 21 days after inoculation of 10% composite bacterial agent were both 1.6 times higher than those in the 10% activated sludge test group 50 Under the high temperature, the total biogas output of the test group inoculated with 10% of compound bacteria agent within 21 days was 2.7 times that of inoculation 10% activated sludge test group and the total methane production was 2.8 times, regardless of the low temperature 20 ℃ or the high temperature 50 ℃, The compound bactericide can significantly improve the efficiency of biogas produced by anaerobic fermentation and shorten the methane production process, providing a new technical approach for the optimal regulation of anaerobic fermentation system.