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目的：观察脑缺血后iNOS基因表达情况。方法：在建立SHRMCAO局灶性脑缺血模型基础上，采用半定量RT－PCR技术观察脑缺血后不同时相iNOSmRNA表达水平变化。结果：显示脑缺血后1h、4h缺血区iNOSmRNA基因无明显改变，12h时iNOSmRNA基因表达升高（P＞0．05），24h、48h后iNOSmRNA基因表达明显增加（P＜0．01）。结论：脑缺血后中、晚期iNOS基因表达增加，其产生的NO可能参与脑缺血后期的继发性病理改变。
Objective: To observe the expression of iNOS gene after cerebral ischemia. Methods: On the basis of established SHRMCAO model of focal cerebral ischemia, semi-quantitative RT-PCR was used to observe the changes of iNOS mRNA expression at different phases after cerebral ischemia. Results: The expression of iNOS mRNA was not significantly changed at 1h and 4h after cerebral ischemia, and the expression of iNOS mRNA increased at 12h (P> 0.05). The expression of iNOS mRNA increased significantly at 24h and 48h (P <0.01) . CONCLUSION: The expression of iNOS gene increases in middle and late stage of cerebral ischemia, and the NO production may be involved in the secondary pathological changes of cerebral ischemia.