论文部分内容阅读
目的:探讨制备方法对莪术醇脂质体包封率的影响,考察其制备的影响因素,优化处方工艺,并对其性状进行表征。方法:采用乙醇注入法将莪术醇制成大单室脂质体(LUV),通过单因素考察,筛选出莪术醇脂质体的最佳处方,优化了制备方法,得到了重现性好、包封率高、简单易行的制备工艺,并对其包封率、粒径、Zeta电位和形态进行综合评价。结果:最终处方莪术醇包封率在70%以上,扫描电镜下观察脂质体为类球状单室结构。用激光散射法测得平均数目径为118 nm。Zeta电位的测定表明脂质体荷负电,约为-31.7 mV。经过加速、光照以及低温留样观察,表明将药物制成脂质体后,与其水溶液相比降解速度减慢,对光的稳定性增加,在安瓶中充氮低温放置6个月,各项指标无显著性变化。结论:乙醇注入法制备的莪术醇脂质体理化性质良好。
OBJECTIVE: To investigate the effect of preparation method on the encapsulation efficiency of curcumol liposomes, investigate the influencing factors of preparation, optimize the formulation process and characterize its properties. Methods: The single chamber liposomes (LUV) were prepared by ethanol injection, and the best prescription of curcumol liposomes was screened by single factor test. The preparation method was optimized and the results showed good reproducibility, Encapsulation efficiency is high, simple and easy preparation process, and its encapsulation efficiency, particle size, Zeta potential and morphology were evaluated. Results: The encapsulation rate of the final curcumol was over 70%. The morphology of the liposomes was observed as a spherical single cell structure under scanning electron microscope. The average diameter measured by laser light scattering was 118 nm. Zeta potential measurements showed that the liposomes were negatively charged, about -31.7 mV. After accelerated, light and low-temperature samples observed, indicating that the drug liposomes, compared with its degradation slowed down the aqueous solution, the stability of the light increased, the bottle filled with nitrogen at low temperature for 6 months, the No significant change in indicators. Conclusion: The curcumol liposome prepared by ethanol injection has good physical and chemical properties.