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目的比较两种不同的膀胱粘膜损伤方法对细胞移植法原位膀胱癌模型建立的影响及优缺点。方法4～6周龄裸鼠两组,每组10只。麻醉后经尿道置入膀胱静脉留置针,导尿后一组注入100ul10%胰酶保留30min,另一组注入100ul0.1mol·L-1Hcl15s,PBS冲洗后将浓度为1×107L-1的人膀胱癌T24细胞悬液注入两组鼠膀胱保留1～2h。另设一组5只导尿冲洗后直接注入细胞保留1～2h。定期观察裸鼠一般情况。28d后断颈处死,解剖观察膀胱成瘤及转移情况,并取膀胱及可疑肾脏行病理学检查。结果胰酶破坏组到2w有2只间断出现淡红色血尿,下腹肿块不明显。到28d后处死,肉眼2只成瘤,约占膀胱50%以上。病理检查证实成瘤2只,多局限于粘膜,未突破肌层。盐酸破坏组约12d开始出现血尿,其中4只可及下腹肿块,2只于2d死亡。到28d处死,共8只大体成瘤,2只见右肾肿大。病理检查示8只成瘤,细胞结构紊乱,未突破浆膜层,两只肾转移。未行粘膜破坏组无明显变化,也无瘤形成。结论胰酶破坏膀胱粘膜对动物损伤轻,但成瘤率低;盐酸破坏粘膜对动物损伤稍大,但成瘤率满意,在细胞移植法建立原位膀胱癌模型可明显提高成瘤率。
Objective To compare the advantages and disadvantages of two different bladder mucosal injury methods in establishing a model of orthotopic bladder cancer cell transplantation. Methods Four to six-week-old nude mice were divided into two groups, 10 in each. Anesthesia transurethral catheterization of the bladder vein catheterization, a group of catheterization after infusion of 100ul10% pancreatic enzyme retention 30min, the other group injected 100ul0.1mol · L-1Hcl15s, PBS rinse the concentration of 1 × 107L-1 human bladder Cancer T24 cell suspension was injected into the two groups of mice for 1 ~ 2h. Another set of 5 catheterization rinsed directly into the cells to retain 1 ~ 2h. Regular observation of nude mice in general. After 28 days, the neck was sacrificed and the bladder tumor and metastasis were observed. Pathological examinations of bladder and suspicious kidney were performed. Results pancreatic enzyme damage group to 2w 2 intermittent pale red hematuria, abdominal mass was not obvious. To 28d after the death, the naked eye 2 tumor, accounting for more than 50% of the bladder. Pathological examination confirmed tumor formation 2, more confined to the mucosa, did not break the muscle. Hydrochloride damage group about 12d began to hematuria, of which 4 and the lower abdomen mass, 2 died at 2d. To 28d executed, a total of 8 general tumor, 2 see the right kidney enlargement. Pathological examination showed 8 tumor, cell structure disorder, did not break serosal layer, two kidney metastases. No mucosal damage group no significant change, also no tumor formation. Conclusion Pancreatic mucosal damage of bladder mucosa is less harmful to animals, but the rate of tumorigenesis is lower. The injury of mucosa by hydrochloric acid is slightly larger but the rate of tumorigenesis is satisfactory. Establishing in situ bladder cancer model by cell transplantation can significantly improve tumor formation rate.