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目的研究南方红豆杉(Taxus chinensis var.mairei)枝叶的化学成分。方法采用反复硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱、高效液相制备柱色谱等方法进行分离纯化;运用现代波谱技术确定化合物的结构。结果从南方红豆杉枝叶甲醇提取物的氯仿萃取部分分离得到13个紫杉烷类化合物和1个非紫杉烷类化合物,分别鉴定为三尖杉宁碱(cephalomannine,1)、9α,10β,13α-triacetoxy-5α-cinnamoyoxytaxa-4(20),11-diene(2)、2-deacetoxytaxinine J(3)、13-cinnamoylbaccatin III(4)、7β,9α,10β,13α,20-pentaacetoxy-2α-benzoyloxy-4α,5α-dihydroxytax-11-ene(5)、2-deacetoxydecinnamoyl taxinine J(6)、Taxin B(7)、2α,7β,13α-triacetoxy-5α,10β-dihydroxy-9-keto-2(3-20)abeo-taxane(8)、taxuspine B(9)、taxachitriene A(10)、taxcuspine X(11)、7-epi-10-deacetyl taxol(12)、N-Methyltaxol C(13)、芹菜素(apigenin,14)。结论化合物6、9、12、13、14为首次从南方红豆杉(Taxus chinensis var.mairei)中分离得到,化合物7、8为首次从南方红豆杉枝叶分离得到。
Aim To study the chemical constituents of Taxus chinensis var. Mairei branches and leaves. Methods The compounds were isolated and purified by repeated silica gel column chromatography, ODS column chromatography, Sephadex LH-20 gel column chromatography and high performance liquid column preparative column chromatography. The structures of the compounds were confirmed by modern spectroscopy. Results Thirteen taxanes and one non-taxane were isolated from the chloroform extract of the methanol extract of the branches and leaves of Taxus chinensis. They were identified as cephalomannine (1), 9α, 10β, 13α-triacetoxy-5α-cinnamoyoxytaxa-4 (20), 11-diene (2), 2- deacetoxytaxinine J (3), 13- cinnamoylbaccatin III (4), 7β, 9α, 10β, 13α, 20- pentaacetoxy-2α- Benzoyloxy-4α, 5α-dihydroxytax-11-ene (5), 2-deacetoxydecinnamoyl taxinine J (6), Taxin B (7), 2α, 7β, 13α-triacetoxy- 3-20) abeo-taxane (8), taxuspine B (9), taxachitriene A (10), taxuspine X (11), 7-epi- 10 -deacetyl taxol (12), N-Methyltaxol C Apigenin (14). Conclusion Compounds 6, 9, 12, 13 and 14 were isolated from Taxus chinensis var. Mairei for the first time. Compounds 7 and 8 were isolated from the branches and leaves of Taxus chinensis for the first time.