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作者采用原发性高血压患者的离体动脉血管 ,并分离、培养动脉平滑肌细胞 ,观察了异搏定、尼卡地平对人动脉平滑肌细胞内钙的影响。由于原发性高血压患者动脉血管平滑肌细胞内钙的跨膜流动和细胞内释放机制尚不清楚 ,作者试图得到异搏定、尼卡地平对人离体动脉平滑肌细胞内钙释放和转运的直接证据。结果如下 :(1)原发性高血压患者动脉平滑肌细胞内总钙比正常血压组高 ,并与细胞内游离钙明显相关 ;(2 )两个钙通道拮抗剂均能显著减少两组的细胞内钙。异搏定显著减少4 5Ca2 +的跨膜内流 ,尼卡地平主要是阻止细胞内钙的释放
The authors used isolated arterial blood vessels from patients with essential hypertension and isolated and cultured arterial smooth muscle cells. The effects of verapamil and nicardipine on intracellular calcium in human arterial smooth muscle cells were observed. Since the mechanism of intracellular Ca2 + transmembrane flux and intracellular release of calcium in the arterial vascular smooth muscle cells of patients with essential hypertension is not clear, the authors attempted to obtain a direct and indirect benefit of nicorandil on the release and transport of calcium from human isolated arterial smooth muscle cells evidence. The results were as follows: (1) The total calcium in arterial smooth muscle cells in patients with essential hypertension was significantly higher than that in normal blood pressure group and significantly correlated with intracellular free calcium; (2) Both calcium channel antagonists significantly reduced the cells in both groups Internal calcium. Verapamil significantly reduces the transmembrane influx of 45Ca2 +, which is mainly to prevent the release of intracellular calcium