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目的:探讨3号染色体常见单核苷酸多态性(SNP)与中国前列腺癌(PCa)发病风险的关系,并探讨其与临床相关危险因素的关系。方法:采用病例对照设计方法,选取124例PCa患者以及年龄、性别匹配的111例正常对照人群作为研究对象,使用聚合酶链反应-高分辨熔解曲线(PCR-HRM)技术结合测序验证法检测SNPrs10934853和rs2660753基因型与等位基因分布情况,分析两位点危险基因型的累积效应,并探讨其不同基因型与PCa患者临床相关危险因素间是否存在相关性。结果:①rs10934853的基因型AA、CC、AC在PCa组中分别有28例(22.8%)、46例(37.4%)、49例(39.8%),在正常对照组中分别有24例(22.0%)、34例(31.2%)、51例(46.8%);rs2660753的基因型AA、GG、AG在PCa组中分别有13例(11.0%)、59例(50.0%)、46例(39.0%),在正常对照组中分别有9例(8.8%)、47例(45.6%)、47例(45.6%),2个SNP的基因型频率与等位基因频率在2组间的分布均无显著性差异(P值分别为0.520、0.582)。②SNP rs10934853和rs2660753两独立变异危险基因型累积效应分析发现:与野生型组相比较,含有危险基因型的两组PCa的发病风险略有增加(OR值分别为1.831、1.968),但组间差异不具有统计学意义(P>0.05)。③SNP rs10934853和rs2660753的不同基因型与PCa患者不同临床相关危险因素间均无显著相关性(P>0.05)。结论:SNP rs10934853和rs2660753与中国PCa的发生无明显相关性,可能与中国PCa的发病风险无关。
Objective: To investigate the relationship between the common SNP on chromosome 3 and the risk of PCa in China and to explore its relationship with clinically relevant risk factors. Methods: A case-control design method was used to select 124 PCa patients and 111 normal control subjects of age and sex matched as control group. PCR-HRM and sequencing were used to detect SNP rs10934853 And rs2660753 genotype and allele distribution, analyze the cumulative effect of risk genotypes at two loci, and whether there is correlation between different genotypes and clinical risk factors in PCa patients. Results: (1) The genotypes AA, CC and AC of rs10934853 were found in 28 cases (22.8%), 46 cases (37.4%) and 49 cases (39.8%) in PCa group respectively. ), 34 cases (31.2%) and 51 cases (46.8%). The genotypes AA, GG and AG of rs2660753 in PCa group were 13 (11.0%), 59 (50.0%) and 46 (39.0% ), There were 9 cases (8.8%), 47 cases (45.6%) and 47 cases (45.6%) in the normal control group respectively. There was no distribution of genotype frequency and allele frequency between the two SNPs in the two groups Significant differences (P values were 0.520,0.582). (2) Cumulative effect analysis of risk genotypes of SNPs rs10934853 and rs2660753 found that the risk of PCa increased slightly (OR = 1.831 and 1.968, respectively) compared with wild-type group, but there was no significant difference between the two groups Not statistically significant (P> 0.05). (3) There was no significant correlation between different genotypes of SNPs rs10934853 and rs2660753 and different clinically relevant risk factors in PCa patients (P> 0.05). Conclusion: SNPs rs10934853 and rs2660753 have no significant correlation with the occurrence of PCa in China, which may not be related to the risk of PCa in China.