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以ＨＩＶ－１ ｐ２４ｇａｇ 与ｈＩＬ－６ 基因在重组痘苗病毒中的共表达研究为目的，将痘苗病毒复制非必需区血凝素（ＨＡ） 基因作为侧翼，把编码人白细胞介素６（ｈＩＬ－６）的基因片段克隆到真核表达质粒ｐ１６ＱＦ１ －２４ 的下游，构建成含有ＨＩＶ－ １ｐ２４ｇａｇ 和ｈＩＬ－６ 两种外源基因的重组表达质粒ｐ１６ＱＦ２４ －ＩＬ６ ，经同源重组和血凝素阴性空斑筛选，获得了重组痘苗病毒ｖ１６ＱＦ２４ －ＩＬ６．经间接免疫荧光试验、Ｄｏｔ－ ＥＬＩＳＡ 和Ｗｅｓｔｅｒｎ ｂｏｌｔ 等检测证明，重组病毒能同时表达ｐ２４ｇａｇ 蛋白和ＩＬ－６ 蛋白，表达产物的相对分子质量分别为２－４×１０４ ，２－６×１０４ ．此种方式表达的外源蛋白持续释放，可产生更强的免疫效果，可望用于ＨＩＶ疫苗的研究．
In order to study the co-expression of HIV-1 p24gag and hIL-6 gene in recombinant vaccinia virus, the non-essential hemagglutinin (HA) gene of vaccinia virus was used as a flanking region and the gene encoding human interleukin 6 (hIL-6 ) Was cloned downstream of the eukaryotic expression plasmid p16QF1 -24 to construct a recombinant plasmid p16QF24-IL6 containing two exogenous genes, HIV-1p24gag and hIL-6. The recombinant plasmid p16QF24-IL6 was identified by homologous recombination and hemagglutinin-negative plaque After screening, the recombinant vaccinia virus v16QF24-IL6 was obtained. The results of indirect immunofluorescence assay, Dot-ELISA and Western bolt showed that the recombinant virus could express p24gag protein and IL-6 protein at the same time. The relative molecular mass of the expressed product were 2-4 × 104 and 2-6 × 104, respectively. The sustained release of the foreign protein expressed in this way can produce a stronger immune effect and is expected to be used in the research of HIV vaccine.