【摘 要】
：
背景:实时定量PCR技术在微生物包括分枝杆菌检测领域应用越来越广泛.目的:评价在痰标本涂片抗酸染色无法获得诊断信息时(如没有痰或痰涂片阴性),RT-PCR在结核病诊断中的作用.
【出 处】
：
国际结核病与肺部疾病杂志（中文版）
论文部分内容阅读
背景:实时定量PCR技术在微生物包括分枝杆菌检测领域应用越来越广泛.目的:评价在痰标本涂片抗酸染色无法获得诊断信息时(如没有痰或痰涂片阴性),RT-PCR在结核病诊断中的作用.设计:我们分析了从2006年1月至2008年4月之间连续纳入的为诊断结核病而进行的支气管分泌物RT-PCR检测的病人.所有病人均接收了计算机断层扫描(CT)、支气管镜和组织活检确诊结核病,对收集的支气管分泌物进行了微生物学和RT-PCR检测.最终的确诊基于微生物学或临床病理学的标准.结果:有136例患者最终得到了诊断结果,其中77例(包括65例培养阳性病例)诊断为结核.RT-PCR在确诊病人中的阳性率是51.9%(40/77).与支气管分泌物抗酸染色相比,更多的病人(20.8%)通过RT-PCR被检测出来(40比20,P
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