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目的探讨老龄cTnIR146W转基因小鼠心脏结构与功能。方法应用GEVivid7超声诊断仪,检测老龄cTnIR146W小鼠心脏结构与功能,包括室间隔厚度(IVS)、射血分数(EF)、短轴缩短率(FS),并用组织多普勒测量二尖瓣环收缩期峰值速度(S)等指标。结果老龄cTnIR146W基因型阳性(A)组小鼠IVS为(1.010±0.011)mm,显著高于基因型阴性(B)组小鼠的(0.911±0.007)mm(P<0.01);A组小鼠EF和FS分别为(71.534±2.488)%和(35.289±1.918)%,均显著低于B组小鼠的(77.737±1.990)%和(40.501±1.697)%(P<0.01);A组小鼠二尖瓣前叶、后叶瓣环收缩期峰值速度分别为(0.032±0.006)m/s和(0.031±0.010)m/s,均显著低于B组小鼠的(0.037±0.005)m/s和(0.039±0.006)m/s(P<0.05)。结论老龄cTnIR146W基因型阳性小鼠室间隔心肌增厚,左室收缩功能减退。超声心动图及组织多普勒可以评价转基因小鼠心脏结构与左室收缩功能。
Objective To investigate the cardiac structure and function of aged cTnIR146W transgenic mice. Methods The structure and function of heart in old cTnIR146W mice were detected by GEVivid7 sonography, including IVS, EF, and shortening of short axis (FS). Tissue Doppler was used to measure mitral valve annulus Systolic peak velocity (S) and other indicators. Results The IVS of aged cTnIR146W genotype (A) group was (1.010 ± 0.011) mm, significantly higher than that of genotype negative (B) group (0.911 ± 0.007) mm (P <0.01) EF and FS were (71.534 ± 2.488)% and (35.289 ± 1.918)%, respectively, which were significantly lower than those in group B (77.737 ± 1.990% vs 40.501 ± 1.697%, P <0.01) The systolic peak velocities of mitral valve anterior lobe and posterior lobe ring were (0.032 ± 0.006) m / s and (0.031 ± 0.010) m / s respectively, which were significantly lower than those in group B (0.037 ± 0.005) m / s and (0.039 ± 0.006) m / s (P <0.05). Conclusions Senile cTnIR146W positive mice have thickening of ventricular septal myocardium and diminished left ventricular systolic function. Echocardiography and Tissue Doppler can assess cardiac structure and left ventricular systolic function in transgenic mice.