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目的探讨p,p’-DDE对离体培养的大鼠睾丸支持细胞(Sertoli cell)细胞周期及凋亡的影响。方法实验采用了从大鼠睾丸组织中分离的支持细胞进行离体原代培养,染毒剂量分别为10、30和50μmol/L,通过流式细胞仪测定细胞凋亡情况。结果在凋亡百分比中高剂量组(50μmol/L)与溶剂对照组比较,差异有统计学意义(P<0.05),30和50μmol/L与溶剂对照组比较在G1、S、G2/M期都有显著的变化(P<0.05)。结论浓度为50μmol/L的p,p’-DDE对支持细胞周期有影响,可能引起细胞凋亡。p,p’-DDE可能诱导支持细胞凋亡,影响细胞周期,最终可能影响生殖功能。
Objective To investigate the effect of p, p’-DDE on the cell cycle and apoptosis of Sertoli cells cultured in vitro. Methods The primary cultured cells were isolated from the testicular tissue of rats by exposure to 10, 30 and 50 μmol / L, respectively. The apoptosis of the cells was detected by flow cytometry. Results The percentage of apoptotic cells in the high dose group (50μmol / L) was significantly higher than that in the solvent control group (P <0.05). Compared with the solvent control group at 30 and 50μmol / L, There was a significant change (P <0.05). Conclusion p and p’-DDE at a concentration of 50 μmol / L may affect the cell cycle and may induce cell apoptosis. p, p’-DDE may induce apoptosis of supportive cells, affect the cell cycle and may eventually affect reproductive function.