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目的检测双嘧达莫对K562人慢性髓原白血病细胞的细胞生长的影响,观察药物作用后肿瘤细胞形态的变化,通过测定药物对细胞凋亡的影响探讨其作用机制。方法用CASY细胞计数仪测定DPM作用于细胞24和48 h,10、20、40和60μg/ml剂量组对K562细胞活细胞数目、活率及平均直径体积的影响,并在倒置显微镜下观察不同剂量药物作用后的细胞形态,采用流式细胞术测定细胞凋亡率。结果双嘧达莫对K562细胞的生长有明显的抑制作用,且有较好的剂量-效应及时间-效应关系。低剂量组仅使活细胞数目减少,而高剂量组可使活细胞数及活率均显著减少(P<0.01),细胞的平均直径与体积也相应减小。与对照组相比,镜下观察可见细胞分布稀疏,细胞浆内出现大小空泡,并产生凋亡小体。60μg/ml双嘧达莫作用24 h可以使K562细胞凋亡早期细胞数量明显增加(P<0.01),达13.2%。结论双嘧达莫具有抑制K562细胞生长的作用,即抑制细胞增殖并杀死细胞,并使细胞形态发生改变,高剂量的双嘧达莫对细胞凋亡具有诱导作用。
Objective To investigate the effect of dipyridamole on the cell growth of K562 human myeloid leukemia cells and to observe the changes of tumor cell morphology after the drug treatment. The mechanism of the effect of the drug on apoptosis was explored. Methods The cell number, viability and mean diameter of K562 cells treated with DPM at 24, 48 and 48 h were measured by CASY cytometer and observed under inverted microscope Dose drug effect after the cell morphology, the use of flow cytometry apoptosis rate. Results Dipyridamole significantly inhibited the growth of K562 cells and had better dose-effect and time-effect relationship. The low dose group only reduced the number of living cells, while the high dose group could significantly reduce the number of viable cells and viability (P <0.01), and the average diameter and volume of cells decreased accordingly. Compared with the control group, microscopically observed cell distribution is sparse, the size of vacuoles in the cytoplasm, and the formation of apoptotic bodies. The effect of 60μg / ml dipyridamole for 24 h could significantly increase the number of apoptotic K562 cells in early stage (P <0.01), up to 13.2%. Conclusion Dipyridamole can inhibit the growth of K562 cells by inhibiting cell proliferation, killing cells and changing cell morphology. High doses of dipyridamole can induce cell apoptosis.