论文部分内容阅读
目的:研究激活补体对血小板的作用和随后对血管内皮细胞和血管平滑肌细胞的作用。方法:用酵母多糖A激活补体观察血小板变形、凝血活酶表达和膜粘度的改变,观察酵母多糖A处理的富血小板血浆对内皮细胞和血管平滑肌细胞的生长、DNA含量和膜微粘度的影响。结果:酵母多糖A能引起富血小板血浆的血小板明显变形、膜微粘度增加和凝血活酶表达增加,但不引起经洗脱的血小板变形,新鲜的贫血小板血浆能恢复酵母多糖A引起的作用,但经眼镜蛇毒因子预处理后作用消失,酵母多糖A引起的血小板变形能被依他酸5mmol/L、Mn~(2+) 10nmol/L、河豚毒素40μmol/L或吲哚美辛100μmol/L阻断,酵母多糖A处理的富血小板血浆上清液能抑制血管内皮细胞生长,但对血管平滑肌细胞生长有促进作用,促使血管平滑肌细胞进入G_2期和M期,同时内浆网变粗和游离的核糖体出现,而内皮细胞的线粒体出现空泡。结论:激活补体引起血小板显著变形,损伤血管内皮细胞并使血管平滑肌细胞增殖。
PURPOSE: To investigate the effects of complement activation on platelets and their subsequent effects on vascular endothelial cells and vascular smooth muscle cells. Methods: The changes of platelet deformity, thromboplastin and membrane viscosity were observed by activating complement of zymosan A. The effects of zymosan A-treated platelet on the growth, DNA content and microviscosity of endothelial cells and vascular smooth muscle cells were observed. Results: Zymosan A could cause platelet-rich plasma platelets to deform obviously, increase the micro-viscosity of membrane and increase the expression of thromboplastin, but did not cause the elution of platelet. The fresh platelet-rich plasma could restore the effect of Zymosan A, However, after pretreatment with cobra venom factor, the effect disappeared and platelet deformation caused by Zymosan A could be inhibited by 5 mmol / L emodin, 10 nmol / L Mn 2+, 40 μmol / L tetrodotoxin or 100 μmol / L indomethacin Blocking and TPA-treated platelet-rich plasma supernatant inhibited the growth of vascular endothelial cells, but promoted the growth of vascular smooth muscle cells and promoted the vascular smooth muscle cells to enter G2 phase and M phase. At the same time, the plasmodesmachy and free Of the ribosomes appear, while the endothelial cells are vacuolar mitochondria. Conclusion: Activation of complement causes significant platelet degeneration, vascular endothelial cell injury and vascular smooth muscle cell proliferation.