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目的探讨亚慢性钐暴露对小鼠睾丸组织抗氧化能力的影响。方法将50只初断乳的清洁级ICR雄性小鼠随机分成5组,分别为对照(蒸馏水)组和5、50、500、2 000 mg/L硝酸钐染毒组,每组10只。采用自由饮水方式进行染毒,连续染毒90 d。检测睾丸匀浆中氧化损伤指标超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)活力和丙二醛(MDA)含量。结果与对照组比较,2 000 mg/L硝酸钐染毒组小鼠睾丸匀浆中SOD、GSH-Px活力以及500、2 000 mg/L硝酸钐染毒组小鼠睾丸匀浆中T-AOC活力均明显下降,500、2 000 mg/L硝酸钐染毒组小鼠睾丸匀浆中MDA含量明显上升,差异有统计学意义(P<0.05)。结论亚慢性钐暴露对小鼠睾丸抗氧化能力具有一定的抑制作用。
Objective To investigate the effect of sub-chronic exposure to samarium on the antioxidant capacity of testis in mice. Methods Fifty clean-weaned ICR male mice were randomly divided into 5 groups: control (distilled water) group and 5,50,500,2 000 mg / L samarium nitrate group, 10 rats in each group. Using free drinking water for exposure, continuous exposure 90 d. The contents of SOD, GSH-Px, T-AOC and malondialdehyde (MDA) in the testis homogenate were measured. Results Compared with the control group, SOD and GSH-Px activity in the testis homogenates of mice exposed to 2 000 mg / L Sm3 + nitrate and T-AOC in testis homogenates of 500 and 2000 mg / L Sm3 + (P <0.05). The content of MDA in testis homogenate of 500 and 2 000 mg / L samarium nitrate groups increased obviously. The difference was statistically significant (P <0.05). Conclusion Subchronic samarium exposure can inhibit the antioxidant capacity of testis in mice.