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通过信息学方法对植物病原真菌分泌蛋白组进行预测以及对碳水化合物酶类CAZymes注释和比较,分析病原真菌寄生性与CAZymes家族功能的关系。结果显示非专性寄生(半活体营养型和死体营养型)真菌编码的分泌蛋白占基因组编码基因的比例较专性寄生真菌(活体营养型)的偏高;CAZymes家族中,非专性寄生真菌的糖基水解酶家族GH和多糖裂解酶家族PL较专性寄生真菌显著扩增;功能聚类分析发现,非专性寄生真菌参与纤维素、果胶、木聚糖等植物细胞壁组分降解相关的基因较专性寄生真菌明显增多。结果表明了CAZymes与寄生类型的高度关联性,揭示了其在非专性寄生真菌致病过程中可能的关键作用。
The informative method was used to predict the proteome secreted by plant pathogenic fungi, and to annotate and compare carbohydrate enzymes CAZymes. The relationship between pathogenic fungi parasitism and CAZymes family function was analyzed. The results showed that the percentage of secretory proteins encoded by the fungi of non-obligate parasitism (semi-biotrophic and dead-vegetative) accounted for more genomic genes than that of the obligate parasitic fungi (biotrophic); in the CAZymes family, non-obligate parasitic fungi Glycosyl hydrolase family GH and Polysaccharidase family PL were significantly amplified. The functional cluster analysis showed that the non-specific parasitic fungi involved in the degradation of plant cell wall components such as cellulose, pectin and xylan More genetically parasitic fungi significantly increased. The results show that CAZymes highly correlates with parasitic types and reveals its possible role in the pathogenesis of non-obligate parasitic fungi.