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研究了高效液相色谱法(HPLC法)检测金银花凉茶饮料中绿原酸含量的方法。采用C18柱(4.6 mm×250 mm,5μm),在检测波长327 nm、流动相乙腈-0.4%磷酸溶液(12:88)、流速1 m L/min、进样量20μL、柱温30℃的色谱条件下进行试验研究。结果表明:金银花凉茶饮料中的绿原酸含量在0.4~102μg/m L范围内线性关系良好(r=0.9999),平均回收率为99.87%,RSD=1.44%(n=9),所建立的方法精密度、稳定性、重复性和准确度均良好,可用于金银花凉茶饮料中绿原酸含量的测定。
A high performance liquid chromatography (HPLC method) was developed for the determination of chlorogenic acid in the honeysuckle herbal tea beverage. A C18 column (4.6 mm × 250 mm, 5 μm) was used at the detection wavelength of 327 nm. The mobile phase was acetonitrile-0.4% phosphoric acid solution (12:88), the flow rate was 1 mL / Chromatographic conditions for experimental study. The results showed that the linear relationship between chlorogenic acid content in honeysuckle herbal tea beverage was 0.4 ~ 102μg / m L (r = 0.9999), the average recovery was 99.87%, RSD = 1.44% (n = 9) The method has good precision, stability, repeatability and accuracy and can be used for the determination of chlorogenic acid in the honeysuckle herbal tea beverage.